Drnigam

Visiting WCHR 2013, UK to improve on HM – Agenda @Dr. Nigam’s My agenda for 7th World Congress on Hair Research, 4th – 6th May 2013 would be seeking answer for following questions by meeting concerned researchers and discussing with them during their presentations. Let me see if I can get video interviews, discussions for you all. All my member friends can also post your questions related to stem cell hair multiplication, DP Cells Injection, DP culture, etc which I might have missed. I will be in Edinburgh from 3rd – 6tth May & 10th & 11th May 2013, I will in London from 7th – 9th May 2013. One member from Netherland will be coming to attend the conference, he is a bio-tech and has been helping me a lot may be I will sponsor him partly. Questions:- 1. Hair follicle epithelial and mesenchymal stem cells interaction and communication to create a new hair follicle in-vitro or in-vivo. Would like to discuss more with Jahoda regarding his organ germ method culture for the same. 2. Trichogenic DP culture, Cohesive DP Structure, In capsulated DP Cells, ECM secreting aggregates of DP cells. 3. How to increase the healing of bisected follicle without the Dermal Papilla? 4. How potent are freshly isolated DP cells from body hair kept in DP culture medium? 5. The in-vitro follicle bisection, what is the minimum requirement for re growth in the bisected follicle (Bulge stem cells + outer root sheath & / or dermal cup sheaths stem cells &/or hair germ matrix epithelial stem cells)? 6. The best method in culture medium for DP culture that is 3D culture hang-in-drop method, how to get a intact basal lamina? 7. Role of repeat injection of Growth Factors in-vivo for activating dormant follicles &/or creating new follicles. 8. Better ways to counter the effects of DHT on Dermal Papilla via TGF-BETA1, how to block or slow down the follicle miniaturization? 9. What difference in hair follicle neogenesis we can expect viv-a-vis multipotent adult hair follicle stem cells vs pluripotent embryonic stem cells? How effective wil be the conversion of multipotent adult hair follicle stem cells to pluripotent embryonic stem cells? How will HM improve if at all by conversion of multipotent adult hair follicle stem cells to pluripotent stem cells and subsequent differentiation? 10. What is my favorite researcher Jahoda’s experience on trans-gender hair transplant and how to achieve optimal results with eye-brow like pluck hair transplant with or without associated Dermal Sheath? 11. How many of these researchers are open to do small clinical experiment on their research findings at Mumbai, India in association with my Clinic & Lab to take advantage of my existing permission regarding stem cell research and software regulations in India? I want to focus on following research papers and subsequent Q&A discussions from 4th – 6th May 2013, Edinburgh, UK:- 1. Update on dermal papilla culture systems Young Wang Sung, Republic of Korea 2. Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches Koh-ei Toyoshima, Japan 3. Classic perspectives on human hair follicle organ culture Michael Philpott, UK 4. Intermediate hair follicles: a more clinically relevant hair follicle model Ben Miranda, UK 5. Tissue Engineering and Regeneration Chairs: Mike Philpott, Bruno Bernard, Kurt Stenn P217 - Full-thickness skin with mature and cycling hair follicles using tissue culture expanded human cells X Wu, L Scott, K Stenn Aderans Research Institute, Inc., Marietta, United States P218 Enhancing hair follicle neogenesis in bioengineered human skin substitutes R Thangapazham1, P Klover1, S Li1, J-A Wang1, Y Zheng2, L Sperling1, G Cotsarelis2, T Darling1 Uniformed Services University of the Health Sciences, Bethesda, United States, 2University of Pennsylvania, Philadelphia, United States P220-Human hair follicle neogenesis using microenvironmentally reprogrammed dermal papilla cells CA Higgins1, CA Jahoda2, AM Christiano1,3 1Dermatology, Columbia University, New York, United States, 2 Biological and Biomedical Sciences, Durham University, Durham, United Kingdom, 3Genetics and Development, Columbia University, New York, United States P221 Calcium microcapsule: a possible scaffold for building artificial dermal papillae C-M Lin1, K Huang2, Y Li3 1Shantou University Medical College, Shantou, China, 2S. Gallicano Dermatologic Institute (IRCCS) Second Affiliated Hospital, Shantou University Medical College, Shantou, China, 3First Affiliated Hospital, Shantou University Medical College, Shantou, China P222 Human scalp derived fibroblasts are multipotent and trichogenic X Wu, K Watkins, K Stenn Aderans Research Institute, Marietta, United States P044 The therapeutic potential of naturally immune privileged hair follicle cells X Wang, J Hao, G Leung, T Breitkopf, Z Ao, G Warnock, J Shapiro, K McElwee University of British Columbia, Vancouver, Canada 8:30-Concurrent Session: 3 Sidlaw Auditorium Stem Cells and Epigenetics Chairs: George Cotsarelis, Vladimir Botchkarev, Denis Headon P186 Topology-dependent plucking leads to more regenerating hairs than plucked via extra-follicular macro-environment modulation C-C Chen1,2,3, L Wang4, TX Jiang1, S Shi4, CM Chuong1 1Pathology, University of Southern California, Los Angeles, United States, 2Department of Dermatology, Taipei Veterans General Hospital, Taipei, Taiwan, Republic of China, 3Department of Dermatology, Yang ming University, Taipei, Taiwan, Republic of China, 4Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, United States P187 Competitive balance of Intra-bulge BMP/WNT signaling reveals a robust gene network governing stem cell homeostasis and cyclic activation E Kandyba1, Y Leung1, Y-B Chen2, R Widelitz3, C-M Chuong3, K Kobielak1 1Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research, Department of Pathology, University of Southern California, Los Angeles, United States, 2Norris Medical Library, University of Southern California, Los Angeles, United States, 3Department of Pathology, University of Southern California, Los Angeles, United States 1030 Plenary Symposium 1: Pentland Auditorium An Update on Hair Follicle Stem Cells Chairs: Satoshi Itami, Rod Sinclair PS1.1 Hair follicle stem cells in alopecia George Cotsarelis, USA PS1.4 Cellular and signaling mechanisms that regulate hair follicle stem cells by live imaging Valentina Greco, USA PS1.2 Mechanisms of hair follicle aging and stem cell regulation Emi Nishimura, Japan PS1.3 Biological and translational potential of hair follicle mesenchymal cells Colin Jahoda, UK 1200 Keynote Lecture Pentland Auditorium K1 Skin Stem Cells: In Silence and in Action Elaine Fuchs, USA 1600 Concurrent Session 5: Pentland Auditorium Androgenetic Alopecia and Hair Restoration Chairs: Ramon Grimalt, Nilofer Farjo, Venkataram Mysore P039 What is the meaning of the “visible pigmented non-vellus hair count” in FDAapproved clinical trial reports? DJJ Van Neste1,2, HD Rushton3 1Skinterface, Tournai, Belgium, 2Brussels’ Hair Clinic, Brussels, Belgium, 3School of Pharmacy & Biomedical Sciences, University of Portsmouth, Portsmouth, United Kingdom P040 Pharmacodynamic of P-3074 (finasteride 0.25% topical solution) in subjects with androgenetic alopecia M Caserini1, R Palmieri1, M Radicioni2, E Terragni2 1Polichem S.A., Lugano, Switzerland, 2Cross Research S.A., Arzo, Switzerland P041 Genetic variations associated with efficacy of dutasteride, the dual 5a-reductase inhibitor in the treatment of male pattern hair loss SJ Jo1,2, A Rhie3,4, S Lee3,4, B-L Lew5, W-Y Sim5, KH Cho1,2, JH Chung1,2, J-I Kim3,4, OS Kwon1,2 1Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea, Republic of, 2Institute of Human-Environment Interface Biology, Seoul National University College of Medicine, Seoul, Korea, Republic of, 3Genomic Medicine Institute, Medical Research Center, Seoul National University, Seoul, Korea, Republic of, 4Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Korea, Republic of, 5Department of Dermatology, Kyunghee University College of Medicine, Seoul, Korea, Republic of P043 Oxidative stress and cell senescence in androgenetic alopecia (AGA) JH Upton1, A Bahta1, N Farjo2, B Farjo2, R Bhogal3, MP Philpott1 1Centre for Cutaneous Research, Queen Mary’s University of London, Whitechapel, United Kingdom, 2Farjo Medical Centre, Manchester, United Kingdom, 3Unilever R&D, Colworth, United Kingdom P227 New development in unlimited hair follicle transplant C-M Lin1, K Huang2, Y Li3 1Shantou University Medical College, Shantou, China, 2Second Affiliated Hospital, Shantou University Medical College, Shantou, China, 3First Affiliated Hospital, Shantou University Medical College, Shantou, China P046 Platelet-rich plasma promoting hair growth in human hair follicle dermal papilla cells associated VEGF and VEGFR-2 BJ Kim1,2, SY Kim1,2, TR Kwon1,2, YH Lee1, YS Song1,2, K Li1, YW Lee3, CH Huh4, CK Hong1 1Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea, Republic of, 2Department of Convergence Medicine and Pharmaceutical Biosciences, Graduate School, Chung-Ang University, Seoul, Korea, Republic of, 3Department of Dermatology, Konkuk University, College of Medicine, Seoul, Korea, Republic of, 4Department of Dermatology, Seoul National University Bundang hospital, Seongnam, Korea, Republic of 1600 Concurrent Session 6: Fintry Auditorium Factors Influencing Hair Growth Chairs: Ryoji Tsuboi, Julie Thornton, Natalia Botchkareva P126 A novel PPARγ modulator (GMG-43AC) inhibits human hair growth and inflammatory response in keratinocytes, and up-regulates expression of epithelial stem-cell associated keratins, K15 and K19 Y Ramot1,2, A Mastrofrancesco3, E Herczeg-Lisztes4, T Biro4, M Picardo3, JE Kloepper2, R Paus2,5 1Department of Dermatology, Hadassah - Hebrew University Medical Center, Jerusalem, Israel, 2Department of Dermatology, University of Luebeck, Luebeck, Germany, 3Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, S. Gallicano Dermatologic Institute (IRCCS), Rome, Italy, 4Department of Physiology, University of Debrecen, Debrecen, Hungary, 5Institute of Inflammation and Repair, University of Manchester, Manchester, United Kingdom P127 Influence of oxygen tension on hair growth and cycle and therapeutic potential of systemic normobaric oxygenation H Kato1, K Kinosheeta1, N Aoi1, K Inoue1, S Itami2, K Yoshimura1 1Department of Plastic Surgery, University of Tokyo Hospital, Tokyo, Japan, 2Department of Regenerative Dermatology Osaka University Graduate School of Medicine, Osaka, Japan P128 Eplerenone stimulates human hair growth ex vivo JE Kloepper1, S Klominek1, T Bir?2, N Farman3, R Paus1,4 P129 Age related expression changes of WNT signaling associated genes in human hair follicle SN Kim1, AR Kim1, SY Lee2, WS Park1 P130 Human scalp hair follicles possess the enzymes to synthesise prostaglandins and prostamides from phospholipids and contain PGFα in vivo KG Khidhir1,2, M Shalbuf1, NP Farjo3, BK Farjo3, JW Wang4, DF Woodward4, SM Picksley1, VA Randall1 P131 Closing KATPATP channels in human hair follicles inhibits hair growth, promotes catagen-like changes and alters paracrine signalling; could this be a new therapeutic approach for excessive hair disorders? H Rahman1, N Farjo2, B Farjo2, V Randall1 P132 Effect of hair follicle environment on nanocarriers stability and drug release properties F Rancan1, S Hadam1, Z Afraz1, B Bellier2, R Wagner3, C Graf4, J Lademann5, E Ruehl4, B Verrier6, U Blume-Peytavi1, A Vogt1 P133 Thyrotropin-releasing hormone (TRH) modulates keratin expression in human skin Y Ramot1,2, G Zhang2, J Hardman3, IS Haslam3, T Biro4, L Langbein5, R Paus2,3 P134 Systemic angiotensinergic stimulation modulates Epithelial-Mesenchymal Transition and collagen deposition in skin and hair follicle ZP Jara1, M Consolo2, SP Panicker2, K Honda2, P Karnik2, S Karnik1 1Cleveland Clinic, Cleveland, United States, 2Dermatology, CWRU, Cleveland, United States 1600 Concurrent Session 7: Sidlaw Auditorium P145 Trichomegaly, or Hollywood “movie lashes” resulting from mutations in FGF5 and an elongated hair cycle anagen phase CA Higgins1, L Petukhova1,2, YY Ho1, E Drill1, L Shapiro3, M Wajid4, AM Christiano1,5 P146 Detection of chronobiological features on the surface of human hair through the multi-stage analysis of Wilhelmy-force profiles P147 Fractions of cortical cell types correlate with macroscopic bending modulus inhuman hair T Itou1, Y Ezawa1, S Nagase1, A Mamada1, S Inoue2, K Koike1 1Kao Corporation, Tokyo, Japan, 2Kao Corporation, Wakayama, Japan P148 In vitro assembly of human hair keratin intermediate filaments: Combination of Keratin proteins and their properties P149 ALOX12 inhibitors promote cuticle maturation that affects on hair Texture K Kizawa1, T Takahashi2, T Fujimori3, R Suzuki2, T Kawai3 1600 Psychology and Hair Disorders Workshop Tinto 1605 Psychodermatology and psychopharmacology of hair loss John Koo, USA Monica Huynh, USA 1800 EHRS General Assembly Kilsyth / Moorfoot 1800 KHRS General Assembly Tinto Monday 6 May 2013 0830 Concurrent Session 10: Sidlaw Auditorium Hair Follicle Development, Control of the Hair Cycle and Pigmentation Chairs: Cheng-Ming Chuong, Emi Nishimura, Desmond Tobin P103 Functional presence of the endocannabinoid system in human hair follicle derived outer root sheath keratinocytes E Herczeg-Lisztes1, IL Szab?1, A Ol?h1, AG 1, N Vasas1, K Sugawara2, R Paus2, T B?r?1 P151 Regulatory T cells increased in the area around and above the bulge of mouse vibrissa follicles in catagen M Oishi, I Niibe, T Matsuzaki Biological Science, Shimane University, Matsue, Japan P152 A meeting of two chronobiological systems: Period1 and BMAL1 modulate the human hair cycle Y Al-Nuaimi1, JA Hardman2, IS Haslam2, T Biro3, BI Toth3, N Farjo4, B Farjo4, M Philpott5, R Watson2, B Grimaldi6, JE Kloepper7, R Paus2,7 P153 Reprogramming regular skin fibroblasts into hair inducing dermal papilla cells C Clavel1,2, A Rezza1,2, M Rendl1,2,3 P154 Development of the mouse dermal fat layer is linked to hair follicle development, occurs independently of subcutaneous fat and is marked by restricted early expression of FABP4 K Wojciechowicz1,2, K Gledhill1, CA Ambler1, C Manning1, CA Jahoda1 P155 ATM expression in hair bulb melanocytes is a marker of oxidative stress in canities-prone scalp SK Sikkink1, O Freis2, L Danoux2, DJ Tobin1 P156 Stem cell factor/c-kit signalling in the hair bulb is essential to maintain hair pigmentation in adult human hair follicles in vivo and in organ culture T Vafaee1,2, TJ Jenner1, SM Picksley1, VA Randall1 1200 John Ebling Lecture Pentland Auditorium K2 Hair Follicle: The Root of the Future. Clinical Research, Implications and Advances Ulrike Blume-Peytavi, Germany CP1 What’s new in clinical hair research? Abraham Zlotogorski, Israel CP2 What’s new in basic science aspects of hair research? Sarah Millar, USA

Recipient growths have been normally good,specially with invitro doubling. This patient is from malaysia ,he will be sending recipient and donor photos every 15 days ,will keep you all updated.

Hi, This is a patient who has undergone in-vivo hair doubling. He has a very poor donor area, mostly single and thin grafts. The patient is from Malaysia and sent this pictures from there as he had purchase a videomicroscope for sending us videomicroscopic pictures. The patient will be coming after 1 month for his multiplied stem cell injection and further doubling of 6000 plus grafts. Till now he has not seen any shedding and the bisected implanted grafts are growing slowly and donor regeneration is also progressing smoothly. Donor Regeneration is up to 80% to 90% by 16 days as he has not send us all the picture of the donor so cant assess it properly. As you can see from the donor regeneration pictures there are no white dots visible and hardly any marks left there. Day 0 & Day 16 click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/B&A/1.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/B&A/2.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/B&A/3.jpg Donor regeneration click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/1.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/2.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/3.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/4.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/5.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/6.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/7.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/8.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day16/Large/Donor/9.jpg

Day-7, Donor Regen & Recep 4417 G Doubing on NW6/7@Dr.Nigam's A case of NW6 (NW7, as per the donor availability and the thin hairs), we plan to make him NW2 with donor doubling, stem cell, DP cell injections. At present we have done donor doubling of 4417 G. In next 3 days we will be doing more 3000 G, 1000 G each day, which brings the total of 7417 G and the same procedure will be repeated after 6 weeks when the patient will return from Malaysia. Recipient Please find below the Day 0 & Day 7 pictures. Today we have done 1495 G by plucked hair transplant without using any hollow needle from donor area. You can see the quick healing & fading away of the marks. Next step: We will be doing Donor Doubling for next 3 days, 1000 grafts each day by plucking method. click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/3.jpg Below is the picture of plucked hair follicles. click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/4.JPG Donor Regen We can see the regeneration of 243 follicles at donor area. We expect 100% donor regeneration in 6 weeks as the follicles grows in 8 weeks maximum. Green dot indicates donor regeneration. click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/4.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/5.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/6.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/7.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/8.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/9.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/10.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/11.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/12.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/13.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/14.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/15.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/16.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/17.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/18.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/19.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/20.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/21.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/22.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/23.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/24.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/25.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/26.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/27.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/28.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/29.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/30.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/31.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/32.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/33.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/34.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/35.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/36.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/37.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day7/Large/Donor/38.jpg

Day 18 - 15 Grafts Patch Test of In-Vitro Hair Doubling @ Dr. Nigam's. Please find below the pictures including comb-over video of Day 18. We can see the regeneration of 90 follicles from 23 follicles Recipient - 38 Follicles (F) Donor - 52 Follicles (F) Recipient Area:- Day 0 & Day 18 Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/Recipient.jpg We can see the Recipient regeneration of Recipient Videoscope Image Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/1.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/2.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/3.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/4.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/5.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/6.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/7.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/8.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/9.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/10.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/11.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/12.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/13.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/14.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Recipient/Large/15.jpg Donor Area Day 0 & Day 18 The surrounding hairs were trimmed for better view of the donor regeneration area. Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/Donor.jpg Recipient Videoscope Image Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/1.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/2.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/3.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/4.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/5.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/6.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/7.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/8.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/9.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/10.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/11.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/12.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/13.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/14.jpg Please click on the link below for the larger view http://www.drnigams.net/images/RTN/Day18/Donor/Large/15.jpg Video Link: Comb-Over Video - Day 18

Please find below the revised counting. Day 0 – 10th April 2013 Recipient – 1251 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/3.jpg Donor – 2268 Stem Cells – 250 DP Cells – 100 Unsuccessful Extraction – 654 click below link to see enlarged close up of the above pic http://www.drnigams.net//images/MS/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net//images/MS/Large/Donor/2.jpg Day 1 – 11th April 2013 Recipient – 832 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/3.jpg Donor – 1272 DP Cells – 100 Unsuccessful extraction – 440 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/2.jpg Day 2 - 12th April 2013 Recipient – 839 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/13-4-2013/Large/2.jpg Used from unsuccessful extraction – 654 + 440 = 1094 Unsuccessful extraction Next procedure will be on Monday (15th April 2013) & Tuesday (16th April 2013) There could be some human error in marking as it has been done by the junior web designer which is a non-technical person. We plant to extract 90% of total donor on monday we will count the total donor left for extraction. By Wednesday we will count from the before picture the no. of single & double graft.

Please find below the revised counting. Day 0 – 10th April 2013 Recipient – 1251 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/3.jpg Donor – 2268 Stem Cells – 250 DP Cells – 100 Unsuccessful Extraction – 654 click below link to see enlarged close up of the above pic http://www.drnigams.net//images/MS/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net//images/MS/Large/Donor/2.jpg Day 1 – 11th April 2013 Recipient – 832 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/3.jpg Donor – 1272 DP Cells – 100 Unsuccessful extraction – 440 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/2.jpg Day 2 - 12th April 2013 Recipient – 839 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/13-4-2013/Large/2.jpg Used from unsuccessful extraction – 654 + 440 = 1094 Unsuccessful extraction Next procedure will be on Monday (15th April 2013) & Tuesday (16th April 2013) There could be some human error in marking as it has been done by the junior web designer which is a non-technical person. We plant to extract 90% of total donor on monday we will count the total donor left for extraction. By Wednesday we will count from the before picture the no. of single & double graft.

Day 0 Before, Day 1 & Day 2 Pics Before Picture click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Before/Large/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Before/Large/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Before/Large/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Before/Large/4.jpg This is patient having a poor donor with thinning. We are doing a new technique called a plucked hair in - vivo donor doubling with addition of stem cells, DP Cells, Growth factor & ECM. Day 0 Recipient 1218 + 831 = 2049 grafts were implanted on the recipient area. click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/3.jpg Donor area 2268 grafts were extracted (you can count from before picture how many single, double) 250 graft were sent to lab for stem cell and dp culture. 100 graft were sent for DP Cell isolation. Day 1 Donor 1272 grafts were extracted from the side of the scalp from the safe zone at the donor and 100 grafts were used for DP cells isolation and injections click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/2.jpg On Day 0 and Day 1 - 654 Grafts were failed extractions ()but grafts not wasted as they will regrow back at donor & 387 grafts were implanted together with other grafts on the same recipient incisions to give density on 4th and 5th line from hairline. We are in the process today should finish in next 2 hours the patient would get approx 1200 grafts today (As all the 9 beds of the clinic were full). ie., day 3 pictures will be post tomorrow. On Day 2 69 donor extraction already showing regrowth, we expect complete donor regrowth to be reuse after 2-3 months after patient return from Malaysia. Donor Video Scope click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/Video_Scope/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/Video_Scope/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/Video_Scope/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Donor/Video_Scope/4.jpg Recipient Video Scope click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/Video_Scope/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/Video_Scope/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/Video_Scope/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day2/Large/Recipient/Video_Scope/4.jpg

Blake, 1)50x magnification

Blake, 1)50x magnification

Blake, For single graft I use 0.5 mm, for double 0.7 mm and for Triple 0.7 mm or 0.8 mm with hollow needle. We are the 1st clinic which uses Ultra Sound guided Partial Follicular Unit extraction. So we can see where we are bisecting it’s not blind technique. It’s a beginning of giving eyes to the Hair Transplant industry.Even fut/fue should be special usg guided. This is In-Vivo doubling plucked hair transplant with stem cells and DP cells. In this case I can go only upto 1mm in the epidermis unlike traditional procedure where they reach epidermis junction, then I tweeze and pluck the graft. As this plucked graft will not have dermal papilla and root so I inject DP cell and after 6 weeks, DP culture + Growth Factor + Stem Cells to repair the bisected follicles.

A case of NW7 for in-vivo Doubling / HM / DP - Day 1 @ Dr. Nigam's This is a special case with poor donor approx Bald area 36 cm x 7 cm = 252 sq cm Donor Area 22 cm x 19 cm = 418 Session 1 (Today - 11th April 2013) – 2268 bisected grafts from Donor to 2268 G to recipient Session 2 (tomorrow - 12th April 2013) – 2000 bisected grafts from Donor to 2000 G to recipient Session 3 (to be done) – 2500 bisected grafts from Donor to 2500 G to recipient In 3 session he will receive 6768 G This patient has received 1st shot of activated stem cells, DP cell & growth factor and will received 2nd shot after 6 weeks. We hope to convert this patient from NW7 to NW2 by 31st Dec 2013 with such a poor donor and unfortunately he is in India only for 15 days. It all depends what sorts of pictures he can provide us but I can provide at least pictures of next 10 days 2268 partial follicular units were extracted (No. of Single / Double will be posted tomorrow after counting) by in-vivo technique with 0.6 mm hollow needle. click below link to see enlarged close up of the above pic http://www.drnigams.net//images/MS/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net//images/MS/Large/Donor/2.jpg Recipient received (on hairline) 1281 grafts (procedure is still on for the remaining graft which will be post tomorrow with no. of single / double grafts) click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Large/Recipient/4.jpg Below are the picture of the donor regeneration:- The procedure is done on day 0 & 601 donor regeneration can be seen on Day 01 click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/4.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/5.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/6.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/7.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/9.jpg'>http://www.drnigams.net/images/MS/Day1/Large/9.jpg'>http://www.drnigams.net/images/MS/Day1/Large/9.jpg'>http://www.drnigams.net/images/MS/Day1/Large/9.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/9.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/MS/Day1/Large/10.jpg

Dear All, 15 G patch test for in-Vitro donor doubling on this MPB case on 28th March 2013. Below are the Before Pictures for 15 grafts patch test including one short video. 6 tattoos have been made on the recipient and 6 tattoos have been made on the donor shaved scalp. Before Pictures click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/01.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/02.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/03.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/04.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/05.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/06.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/07.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/08.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/09.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/10.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/11.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/12.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/13.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/rtn/large/14.jpg Video Link :- Day 0 20130330170528 - YouTube Day 3 Procedure Video - 15 Grafts In-Vitro Hair Doubling procedure Below are the pictures of Day 0 and Day 3 of recipient and donor regeneration. Also find video of the procedure on Day 0 including pictures of graft bisection. click the below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day03/Large/01.jpg click the below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day03/Large/02.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/large/33.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/large/34.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/large/35.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/large/36.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/large/37.jpg Kindly find below 1. Before picture of every extraction and recipient 12th Day picture of every extraction and recipient microscopic large & zoomed picture. Now we can easily follow the case by keeping an eye on the surrounding hair. We have started with 15 G extraction at the donor area (6 Single, 8 Doubling & 1 Triple follicle) total 23 follicles in between 6 tattoo marks. Believe it or not, it is surprise to me too that we can see on 12th day 46 growing follicle at Donor and 34 growing follicles at recipient, regenerating themselves with additions of new follicles formation. Total 80 follicles re-grown from 23 follicles extracted. Remember we have also injected stem cells, dp cells, dp growth factor, ECM + PRP. Hence new follicle regeneration also as a part of our stem cells hair multiplication therapy. In the months to come we will follow the rate of growth and also the thickness of the follicles. Donor Area Day 0 picture for reference click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/donor.jpg Donor extraction Day 12 Microscopic pictures click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/4.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/5.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/6.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/7.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/8.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/9.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/10.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/11.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/12.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/13.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/14.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Donor/15.jpg Recipient Area click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/ricipient.jpg Recipient extraction Day 12 Microscopic pictures click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/1.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/2.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/3.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/4.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/5.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/6.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/7.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/8.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/9.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/10.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/11.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/12.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/13.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/14.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/RTN/Day12/Large/Ricipient/15.jpg

Krishonkrush, Yes, because we now have the misssing link, that is dp cells and dp culture. Now we already have the root, that is dermal papilla ,previously we had only stemcell solution like aderans, hence results were approx. 50%. With this i am expecting 80 to 90% result,what to we mean by 80 to 90% result, i will explain later. I would like you to document your case like in other forums for the community ,it will be revealing experience for all of us. Final treatment plan we will decide after meeting on skype or in person. Kindly go through the case study of in vitro doubling with stemcells and dp cells wherein with full proof documentation we got 80 follicles from 23 extracted from the donor,this was not pure stemcell technique but a combination.(will post this study on hrn tomorrow. I would make you talk to few patients and see their result and share with community since you are from mumbai/india.Most of the community guys are from outside india ,as you are aware. rQUOTE=krishonkrush;2343366]Hi Dr.Nigam, Sorry for late reply. First of all thanks a ton for your reply, it really sounds confident and convincing. I would really like to share my photographs with you. Will be contacting you soon personally either by gmail or skype and share my details. Just need some time to get my hair grew a bit, as i trim my head regularly, and then would share my photographs. So, by saying "We have stopped doing FUE/bht from apri 2013", do you really mean that stem cell could give me good amount of hair and no transplantation would be required at all??

Krishonkrush, Yes, because we now have the misssing link, that is dp cells and dp culture. Now we already have the root, that is dermal papilla ,previously we had only stemcell solution like aderans, hence results were approx. 50%. With this i am expecting 80 to 90% result,what to we mean by 80 to 90% result, i will explain later. I would like you to document your case like in other forums for the community ,it will be revealing experience for all of us. Final treatment plan we will decide after meeting on skype or in person. Kindly go through the case study of in vitro doubling with stemcells and dp cells wherein with full proof documentation we got 80 follicles from 23 extracted from the donor,this was not pure stemcell technique but a combination.(will post this study on hrn tomorrow. I would make you talk to few patients and see their result and share with community since you are from mumbai/india.Most of the community guys are from outside india ,as you are aware. rQUOTE=krishonkrush;2343366]Hi Dr.Nigam, Sorry for late reply. First of all thanks a ton for your reply, it really sounds confident and convincing. I would really like to share my photographs with you. Will be contacting you soon personally either by gmail or skype and share my details. Just need some time to get my hair grew a bit, as i trim my head regularly, and then would share my photographs. So, by saying "We have stopped doing FUE/bht from apri 2013", do you really mean that stem cell could give me good amount of hair and no transplantation would be required at all??

Question mark, It was blessing in disguise, as i thought my answer was posted. So thanks for the post.

Krishon, Krishon and question mark, I am sorry ,i posted the full reply but only two lines were posted may be due to some posting error. I will reply again. I apologize on behalf of my front desk that she did not give you access to me or other doctor at the clinic. We have stopped doing FUE/bht from apri 2013. No more fue hairlines as in the past. I will be able to comment in detail about your case once either i am told whom did you speak to at my clinic ,since i don't know ur real name, this will help me take out your details or if you can mail me your pics dr.rahul1970@gmail.com or can talk to me on my skype id dr.nigams Regarding stemcells,this is the procedure offered at my clinic- 1)Day0 -Extraction of 50 to 100 grafts and few more grafts for dp cell/dp injections and stemcell isolation and culture from your donor scalp or if the donor is weak from chest . We will give you first shot of activated stemcells on day 0 evening with dpcell/dp injection plus growth factors plus ecm plus prp. 2) We will repeat the procedure twice after 6 weeks plus dp culture injection. With this, as of now (with improved technique) you have 70 to 80% chances of success of regrowing new follicles and activation of dormant follicles with minimum density of 30 hair follicles (not graft) per sqcm of injected site. We will keep track with video microscope and macro pics. 30% chances are there, you may not get any hair or few hair that is less than the density i mentioned. Now what should we do if you fall in the category of 30% unfortunate scalps. That is when we offer with the above stemcell procedure ,the donor/hair doubling. Please understand we are moving towards creation of dermal papilla implant and subsequently hair germ or microfollicle in vitro in the lab. Till that time ,it's a good idea to use dermal papilla from chest hair by isolatimg from it or partial follicle by plucking a part of hair follicle from the donor scalp, which acts like a proto hair or a partial hair structure ,which can interact with multiplied stemcells and dp culture to create follicles with near 98% success(theoretically 100%) No more FUT/FUE/BHT at our clinic only STEMCELL AND HAIR DOUBLING. We are hoping by next year mid , we may offer only stemcell therapy for MPB. Please contact me directly on mail,skype or in person. You know the workload at our clinic , and unfortunately i am either in lab or in the procedure and sometimes you don't have access to me till you have joined the procedure.Every patient including you is important. Thats why i am active on forums ,so that i can reach outto my potential patients directly so that for technical details you are not dependent on my counsellors,as my councellors are at least 1 month behind the new therapies i introduce.

Blake, sorry for inconvenience. If you can give me access to edit and reduce the size of pics with link to view enlarged pics or if you could do it for me.

Blake, If you could reduce the size of the images or give me access the modify the post,i will do the needful. QUOTE=Future_HT_Doc;2341537]Dr. Nigam, Again, let's see if we can get the images sized more appropriately so the thread will load. Let me know if you need a hand with this one as well.

We did 2500 grafts Hair Doubling to 5000 grafts on day0. We extracted 2500 Grafts from the donor with 0.5 mm punch and bisected the graft at the level of dermal papilla. Boosted these grafts with progenitor stem cells+ DP cells = ecm+growth factors+prp.As you can see the patient is complete growth at the recipient.All the bisected grafts were implanted at the recipient not at the donor.This patient is adermatologist from delhi . This patient will receive 3000 grafts doubling to 6000 in next 2 days and hence will be a case of 5500 grafts double to 11000 grafts thus will be converting from NW5/6 to NW2/1. He is 27 years old Dermatologist who also perform hair transplant from Delhi, India.Freddie this for you ,we hope e can convert this case fromNW5/6 to NW1/2 after his second session in next two days.NEVERSAYNEVER also listened to my conversation with this patient( doctor) Further pictures after doubling of 3000 grafts to 6000 grafts will be provided next week. Picture 1 Day 0 & Day 11 pics of in vitro hair doubling. This is a Dermatologist & a hair transplant Doctor himself moving towards NW5/6. 2500 G were extracted by 0.5 mm punch & bisected to 5000 G and all the bisected grafts were implanted on the recipient area. Activated progenitor stem cells + DP cell + ECM + PRP + Growth Factor were also injected into the 5000 G Picture 2 Day 0 & Day 11 pics of in vitro hair doubling. Donor scalp on Day 0 & Day 11. Picture 3 Day 0 & Day 11 pics of in vitro hair doubling. This is a Dermatologist & a hair transplant Doctor himself moving towards NW5/6. 2500 G were extracted by 0.5 mm punch & bisected to 5000 G and all the bisected grafts were implanted on the recipient area. Activated progenitor stem cells + DP cell + ECM + PRP + Growth Factor were also injected into the 5000 G Picture 4 Day 0 & Day 11 pics of in vitro hair doubling. Vertex with few bisected grafts, more graft will be implanted in the next session of doubling tomorrow Picture 5 Day 0 & Day 11 pics of in vitro hair doubling. Extracted grafts from the donor scalp

Picture 1- Day 0 477 grafts (mostly single & double) were plucked with fine forceps after loosening with 0.7 mm hollow needle (we can also do plucking with 0.3 to 0.4 mm hollow needle). Around patient birth mark 10 grafts were plucked for follow up. 551 grafts (mostly single & double) were plucked with fine forceps after loosening with 0.7 mm hollow needle (we can also do plucking with 0.3 to 0.4 mm hollow needle) Around patient birth mark 10 grafts were plucked for follow up. Left recipient of Patient immediately after the procedure you can see need bloodless field with 631 plucked grafts were implanted. He still needs more density which could not be done due to shortage of time and the same will be done on his next visit free of cost. You can also see 100% intact implanted follicles on the recipient area. Recipient was also injected with activated, epithelial, dermal cup sheath stem cells & isolated DP cells from chest follicles, ECM, growth factors & PRP. pre-implantation slits were made on the recipient area to avoid prop-out of grafts & keep the whole sticky with granulation tissue. The slits were first injected with PRP & DP cells at the derma sub-cutaneous junction. Right recipient area of Patient , immediately after the procedure you can see neat bloodless field with 673 plucked grafts were implanted. He still needs more density which could not be done due to shortage of time and the same will be done on his next visit free of cost. You can also see 100% intact implanted follicles on the recipient area. Recipient area was also injected with activated, epithelial, dermal cup sheath stem cells & isolated DP cells from chest follicles, ECM, growth factors & PRP. Pre-implantation slits were made on the recipient area to avoid prop-out of grafts & keep the whole sticky with granulation tissue. The slits were first injected with PRP & DP cells at the derma sub-cutaneous junction. Both the recipient areas has 276 extra grafts which were 2 follicles converted to 1 follicle for aesthetic hairline purpose. We are also expecting more grafts on the recipient because of stem cell, DP cells & growth factors injections. Patient had already provided with ECM at the donor and recipient for quick healing and to avoid any mark. I do not claim but Dr. Hitzig and Dr. Gho claims ECM activates hair stem cells but I do claim injury to the plucked follicles will definitely activate stem cells. Less shedding will be seen because the plucked follicles will first try to recuperate and manufacture the other half as against going in a resting phase after the hair transplant.

Blake, I agree with you that next logical step is hair/donor doubling with stemcells and growth factor and dp culture. The problem at present with hair multiplication is we are able to isolate and multiply hair epithelial stem cells and culture dp dermal cells from which a follicle is formed in a mothers womb. But the cross talk and induction of these cells is not in our control in vivo inside the scalp. As we reach to the next step of dermal papilla creation in lab and implantation in the scalp,results will improve by leaps and bound. At present in the fortunate scalps, this cross talk and induction happens and some get great results and some do not get any result. In case of hair/donor doubling we are fortunate to have a proto bisected distal follicle and bisected proximal dermal papilla intact for autologous stemcells to interact with and hence much higher and predictable success rate.

Blake,Before me ,the well known research scientist Jahoda and others have shown in several studies that 70% growth of the bisected follicular units after bisection and reimplantation.As we add to the bisected follicular units progenitor stemcells on day 0 and multiplied stemcells at 6 weeks +dp cells +ecm+prp+growth factors, We started hair doubling 6 months back. We have seen, in last 6 months regrowth of bisected follicular units to be around 90 to95%. Regarding the shaft diameter ,we will measure the same after 6months, as it takes 1 to 11/2 year for even FUT/FUE transplanted hair to achieve full thickness In our 6 months observation the shaft diameter is comparable to traditional FUT/FUE hair around this time.We have the advantage of stemcells and other factors as mentioned above.I will post the pic of 28 days followup of a patient on monday, for whom we have doubled 1150 grafts to 2300 grafts. In another forum i have opened my clinic to FREE patch test for forum members with 30 to 50grafts, for the month of march and april 2013 to those patients who are ready for semi permanent tattoo and are ready to document the same with hair counts,as an independent observer.

Stifffler, When a graft is doubled into two,this is when we call it 100% success as far as the question of doubling is considered (Jahoda and others have conducted hair doubling studies without addition of stem cellls and growth factors with 70%regrowth in both the bisected follicles.Since we add progenitor stemcells and growth factors our success rate is 90 to 95% as on today). When the diameter or the thickness of these bisected two follicles is not less than the original thickness of the follicle,than we can claim to achieve 100% success rate, as far as the quality or character of the follicle is concerned. Rate of growth of these bisected follicles is also a parameter to watch for. These bisected follicles tend to skip the shedding phase after implantation probably due to injury to the follicle and addition of stemcells, hence enters repair phase rather than shedding phase, seen commonly after traditional FUT and fue hair transplant. As you can see in the above case study, the doubling as per as the number is concerned is not an issue, The quality/diametre of hair in our last 6months experience( as we started with hair doubling since last 6 months) is not any different from the thickness achieved by follicles after traditional FUT/FUE transplant, the exact diameter we be measured after 6 months ,when our cases reach 1year growth, as in any transplant the final thickness of the follicle is achieved at 1 to 11/2 year of transplantation. Any case which is an indication for FUT/FUE is fit for hair doubling, specially the MPB patients with poor donor availability at the safe donor zone. We are in the process of converting NW6/7 to NW2 with hair doubling which is not possible with FUT/FUE except with body to hair transplant. 10000 grafts required for the same can be created by 5000 donor grafts doubling(in cases with availability of good donor or the body/ beard grafts need to be used). Hair doubling will also help in giving higher density than FUT/FUE thus more natural result. stuyQUOTE=stifler;2339084]Ahhh, that is a great summary! Thanks! I'm assuming the serum acts as a growth medium. Dr. Nigam, what has been the success rate so far for this (also, if you could specify how you are defining success, that would be great)? For me, success would be that you actually get new hair (doubling) that grows in a sustainable manner. Who are the best candidates for this?

Blake, Lauster's and Dr Gerd created the world's 1st microhair follicle in Vitro in the lab. I have visited their lab in Berlin in the month of Sep 2012. Their team along with Dr Gerd visited my clinic at Mumbai, India this Jan 2013. We had long discussions on technical issues regarding stem cell hair multiplication, dermal papilla culture and implant, proto hair implant. Till the time we perfect HM, hair doubling with stem cells and DP cells is a good option for people with poor donor or insufficient donor to cover their MPB.