Drnigam

SK07, Arashi will keep me busy. wesley never said that his procedure has failed in 4 months. It is Arashi who again started at BT, claiming wesley's procedure, he is good in scaring people.He will come up with one controversy every fortnight...hope so not earlier..thanks for giving my time to rebutt..as i do not post photos at bt,arashi takes advantage..by coming to his own conclusions... Kindly have a look at the before picture's of wesley and also compare the same with after 4 months pictures posted by wesley at BT yesterday. It is been alliged that his donor is depleted without even checking out is shaved donor, as I have extracting only 1024 grafts from the donor scalp.They were bisected in vitro, 1635 bisected grafts were implanted with the receipient and approx 450 grafts were implanted back of the donor scalp. How can 1024 grafts deplete suddenly. It is only when wesley shaves his donor and compares with the before pictures, we can know that exact scenario. Not just because pro hair clinic recommend him scalp micro pigmentations (Although he had scalp micro pigmentations this is mind) 631 grafts were extracted from the beared. 150 grafts sent to the lab. 431+ grafts were implanted at receipients without doubling. 50 grafts were implanted on his FUT scalp as a test procedure for a second session. So that the next session we can fill the FUT scar with beard grafts in the next session. Fees paid only 1600 US $, since he was in boldy's friend that is, cost to cost. Which can be return any time after one year of procedure, if the patient does not get desired result, not before that. Result what should be expected at the end of one year should be as follows. 1. Total 1635 invitro bisected grafts should grow back at the frontal scalp and interior vertex. 2. 431+ beard grafts should grow at his receipient by the end of one year. 3. 631 extraction holes at beard should not show any white dots. Already we can not see 631 blank spots at his beard. Yes density at beard extraction sites as it was mainly normal beard FUE. 4. Even though he has not received any multiplied Stemcells and DP Culture, I am still expecting donor regeneration of more than 450 bisected grafts implanted at the donor. As he was applying nano growth factors, A cell like ECM. To analysed the sucess of his procedure, wesley need to shave his donor every three months, and take pics of beard and receipient and post to us. Kindly also read below, click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/2008.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/2011.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/baard1.jpg Wesley in july end 2013 click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/bt3.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_09.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0012.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0117.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0292.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0294.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0364.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0588.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0640.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/IMG_0649.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/WL/Large/t2.jpg what information wesley has provided at the BT forum 3/4 months back. a quick history of myself : hairloss started with me when i was 18 years... now i am 31 years old... i started meds and first ht in 2008 ( 2700 grafts fut in istanbul) , then after that i did a fue in belgium (2509 grafts). i use alot of meds , and the last 2 years i'm doing bodybuilding as a hobby , no need to say that higher testosteronlevels for musclegrowth are nog so good for people like me with mpb , so to cut this short, my hair took a beating over 2 years time... i was schocked to see my crown , normally i style my hair with bit of fibers also, and it looks always great , i style it 365 days every year , so when i got to see my before pics was a schock to me... so what where my options ? i could not do a big fue , because of my almost done donor... i'm a hard case for clinics because of my low donor left... so i readed more into acell , dp culture and this hairdoubling... verry intresting for people with lower donor, or just people that dont want to thin out there donor. i watched the posts of this hairdoubling on the forums , and contacted dr nigams for this procedure... i arranged vacation on my work , and filled in application for india visum , airplaintickets , and dr nigams arranged me a good hotel not far from the clinic... It seemed Boldy wanted to go there also for his experiment so we staid at same hotel. arrived at mumbai i got picked up at the airport of mumbai by a driver of dr nigams , they drived me to the hotel (4-star svenska hotel) verry nice hotel with options to eat european foods... after a good nightrest , i got a phonecall of dr nigams to arrange a hour of pickup , so we can talk and see what is possible. when arriving in the clinic dr nigams was waithing for me at the reception , he is verry nice and realistic for what is possible and what my expectations are... to save time for the next day they suggest to shave my head for extraction , i asked dr nigams if its possible to keep my hair long on top , they could to the transplant without of shaving on top , so the nurses only shaved the sides and back of the head , so i got a hairstyle that still covers my head on top , to keep it as invisible for my work and friends as possible when i return to belgium... dr nigams showed me his other clinic , so he has 2 clinics in mumbai , and i have seen his testperson in real life with the tatoo-dots on his donor and recipiant for his hairdoubling case studie , and seen also the regrowth on the frontal zone on this testperson with his activated stemcell injections.* dr nigams asked me if i had done a bloodtest , every patient needs to do a bloodtest to check bloodvalue and check for hiv , i have done 2 transplants in the past but i had never been asked to do a hiv bloodtest before , its for patient and staff safety he told me , so its verry safe to do a hairtransplant in india , sinds your first checked in his clinic , before they do any procedure... so his nurses taked some blood for lab-tests , and after this i got back at the hotel to rest some more. the next day , procedure time... i got picked up at the hotel and deliverd to the clinic , here the reception called the lab to know the blood-test results , so this was a bit stressy to see what they find after getting all the bloodtest results everything was good , no hiv thankgod and with the other tests they see if your blood is not to thin or anything else ,everything was good the nurses escorted me to the clinic on the first floor , they applyed prylox on the donor and recepiant area , a local anesthetic so you feel the priks less of the anesthetic-syringes. they also use ice with the injections so you dont feel any pain, after a few pain-checks they started to extract the donorhairs , after a few hours they extracted a total of 1042 grafts that they doubled with cutting under thereoscopic microscope with androidsystem on top (so i could follow it to see how they cut my grafts) i got to eat a bit upstairs , while they complete the doubling procedure, they coock or order whatever you want to eat...* after doubling they had 2084 grafts , to ensure donor recovery and keep backup on my donor for future procedures they put 450 grafts back in the donor.. the remaining 1634 grafts are inplanted in the hairline and frontal zone of the head , to densify the frontal zone more.. also a part of my chesthairs where extracted also , for dp survival , to boost the grafts , they inject activated stemcells with prp and growthfactors in donor and recepiant zone. before i forget , the grafts are injected in the skin , no slits... so no bleeding... and grafts are verry tight deep in the skin , at the end they clean everything and apply ecm cream with growthfactors mixed in and put it on the donor and recepiant zone to boost healing , and apply some bandage to keep donor protected and keep of any swellings in the face. they gave me a spraybottle with growthfactors in it , to spray on the recepiant zone , and some medications : anti-inflammatory , antibiotics and other... the staff on the clinic work hard , the doubling and inplantation between my long hair on top makes it just more difficult and takes some more time to complete , in the evening the driver takes me back to my hotel so i can rest for the procedure next day. next day i had good breakfast in the hotel and got picked up by nigams driver... at the clinic the doctor discuss what my options are , we go for extraction of beardhairs , and double these , to densify the crown and mid section of the head , the first day i allready talked this over also , so i had allready trimmed my beard in the hotel , to safe time... they applyed prylox on the beard and recepiant zones (mid section + crown) after local anesthetic on the beard they started difuse extraction of beardhairs... after extraction they started to double the beardhairs , they told me that its limited this doubling sinds they can only double straith beardhairs , and alot are no suitibel for doubling... so while they doing the doubling i go to the upper floor to eat my orderd chicken sandwich from subway sandwiches... after a good meal i was escorted back to the procedure room , where the staff was waithing to start local anesthetics on the mid section and crown , the extracted hairs ( 631 grafts) where doubled for 200 grafts more , so the total grafts where 831 grafts. the inplantation took a bit longer now sinds they need to follow the crown hairdirections , beardhairs where difuse injected in mid-section and crown area... i didnt had much notice of the hours that goes by sinds i was watching englisch movies that i had chosen ( he has sattelite tv with large choise of englisch movies , all new movies) they also extract more chesthairs for again , graft dp support , and got injections of his activated stemcells with prp and growthfactors.... after the procedure they put on every zone some ecm cream with growthfactors mixed in , to boost healing... it was in the evening and i got drived back at my hotel , i eat there something from the roomservice and talked with my family some , and go to my bed to sleep. the next days i go to the clinic for some checkups, cleaning bandages and for a few injects with prp , growthfactors (not sure if this time there was also activated stemcells, cant remember) i got also some tubes ecm creme with me for in the hotel , i must say that i did not had any pains , not in the procedure , and not after the procedure , so the optional painkillers they gave me i didnt take these , also the healing is spectacular , i had never seen so fast healing , dont know if this is from his stemcells or prp or growthfactors , maybe its the combination that does the trick , also i have seen on my chest that the extracted grafts are recovering, first time i see new hairs growing out of extracted holes... almost 30 extractionpoints on the chest grow new hairs, there many grafts that look thicker and grow faster then hairs that are arround the extracted zone , verry strange... a few hairs i helped to get trough the skin , dont know if i need to do this , or that the hairs over time push trough , but a few holes i push on the skin a bit and i pull hairs out the skin that are 1 cm in length , or even longer... is it with his activated stemcells , or the growthfactors , i have never seen anything like this before... and i had watched them removing the chesthairgrafts , they dubbelchecked at the end , so how is this possible ? you can forget a graft its possible , but i have seen 30 grafts generating new hairs ? so this i my revieuw for now , ill be posting updates with pictures... for now this are my update pictures for now : also have i documented the regrowth hairs on the chest so you see clearly they grow out of the extracted holes... i'm curious what the results will be , if this work , yes or no , time will tell.... ps sorry for my English its not to well. from 300+ extracted hairs , are growing 30 back , with thicker density then the remaining bodyhairs , and they grow faster... also i helped 10 hairs getting trough the skin , dont know if they would go trough over time... dont know why i see here regrowth , i applyed also a high dosed growthfactormix , or is it because the activated stemcell , not sure... but its clear that its new hair , growing out of the extractiondots... I could not be in touch with wesley after the procedure, but boldy was updating his progress to me... Also find few pics sent by boldy through his phone ,hence blurred...you can see good growth in 3/4 months at boldy's temples...wesley and boldy's case was done together. click link below to see enlarged close up of the above pic http://drnigams.net/images/F/Large/1.jpg click link below to see enlarged close up of the above pic http://drnigams.net/images/F/Large/2.jpg [div][postedby]Originally Posted by HMorHT[/postedby] Prohair has a history of attacking Gho and stem cell hair transplants, I do not expect Prohair would have anything good to say about Nigam's results. Let me guess, the scalp pigmentation treatment was offered to you by Prohair?[/div]

Dear sK07, Yes, it all started with this pic.. Dear Future doc,i am posting only doubling and HM pics,as mentioned by you in the earlier post. I have already posted full face photos to prove the allegation false . Have a look yourself at the full face pics..and confirm that the patient in the before and after is the same. Also it is alleged that I said that the before picture is of 7 months before. And the before picture as per photo software picture is two months old. Kindly read my first post regarding this pictures, where I never mentioned that the before picture is 7 months old but I said that after picture is post 7 months of the first injection. Again I do not blame skeptics for doubting when I first posted the same without full face photo,in 7 months.. weight,length of hair, lighting,angle could create doubt. We can only post pics of the patients with full face ,only when the patient allows. They had full right to doubt, only it would had been better had they asked for clarification before they made their judgement. click below link to see enlarged close up of the above pic http://www.drnigams.net/images/DK/1/Large/1.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/1.JPG click below link to see enlarged close up of the above pic http://www.drnigams.net/images/DK/1/Large/6.jpg click below link to see enlarged close up of the above pic http://www.drnigams.net/images/DK/1/Large/5.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/2.JPG click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/3.JPG click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/4.JPG click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/5.JPG click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/6.JPG click below link to see enlarged close up of the above pic http://drnigams.net/images/DK/8Nov/Large/7.JPG Again few doubted, specially the same posters, about this photo also, saying that these two are also different person,until i posted full face photo..to clear the isue. I have refuted every false allegation by 2 particular members ,who were and are after me.. click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/B-A/Large/3.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/8Nov/Large/1.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/8Nov/Large/2.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/8Nov/Large/3.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/8Nov/Large/4.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/8Nov/Large/5.jpg click below link to see enlarged close up of the above pic http://drnigams.net/images/Savio/8Nov/Large/6.jpg

lucidh, With due respect to you, Any comment on anybody or any technique should be made only after giving a chance at least ,to clarify and be heard , specially when I am totally open and transparent. , I have only 3 actual patients namely Mr.Nikhil from Chandigarh, Mr.Viren from Mumbai and Mr.Naveen from Calcutta in the whole of google in my entire practice who have adversely written against my clinic, and kindly note that these patients had not met me directly neither had been treated by myself directly, and as soon as I came to know about these three, I contacted them and have promised them 15000$ free treatment each. This complementary treatment started 4 months back for Mr.Viren, 2 months back for Mr. Nikhil and 1 month back for Mr.Navin. All three are satisfied now and have written the positive reviews after meeting me personally in their respective links. In fact, Mr.Viren have responded well to doubling and hair multiplication treatment both, and I will request all three of them to post on this thread itself regarding their experience after meeting me in person directly. I will post Mr.Viren's photo next week on this thread and will keep you updated with Nikhil and Naveen's progress as their treatment progresses. Nikhil and navin were only few months post treatment, and they did not wait for the growth to happen , before they wrote their comments. Except these 3 patients in the hair category, the rest of the comments made by any poster ever, have never mentioned that they have been my patient, most of them are my competitors writing against my clinic or few might have just enquired on phone or must be opinion makers like you who may read some adverse comments without verifying and make a biased, prejudiced opinion. Of Course, in a democratic platform, everybody has a right to make their opinion. By the way,what you claimed as your research, has been posted by every new member, everytime he comes to these threads... I am doing highest number of hair transplants in India, per month, per clinic. It is but natural, I will have jealous competitors, specially as I bring the new game changing technique "hair doubling." The no.1 FUT hair transplant surgeon of Mumbai just 2 years back, is on the verge of closing down his clinic as on today. The link you provided is nothing... you will see in the coming months and years, more such attacks with ulterior motive to malign and defame my clinic by competitors... as my work become threat to them for there professional survival,it is part of the game,when you come up with something new... Regards, stem cell hair multiplication, I have never claimed that it is 100% successful as on today, but yes, what can I do if it gives excellent results to some patients and no results to others. I have posted the photographs of the same and I am transparent and open by allowing access to these patients for anyone with their names , telephone nos. and email ids or even through skype. Those patients who decide to undergo hair multiplication treatment with me, sign a legal document with my clinic which clearly mentions that the clinic and the patient agrees that hair multiplication is an evolving science. Some patients may get desired results, some may not. In case, the patients does not get the desired results, he will be provided free hair doubling or free FUE against the amount paid by him, If my patients after understanding all the pros and cons of the evolving hair multiplication technique, decide to undergo the same willingly, with their consent, then why should you have a problem without even getting your so called doubts clarified. Regards , hair doubling,i am 100% sure of results(but not expecting you to believe ,yet), i know, what i am doing... Yes, you may take time , may be months, or may be years (the way people did not believe fue when it was introduced 10 years back) to believe, but that is democratically speaking , your problem. You said you are an INDIAN, it was more easy for you to have first communicated with me, and if not convinced, you had a full scientific right to criticize.. Without even knowing me or even talking to me..you seem to have a done a very unbiased scientific research on the techniques.... I do not make opinions by smelling, but by continous thorough scrutiny... Do You think any doctor or even a patient will believe anyone blindly... I never focus attention on my own self or my name.. I always give credit to others , with the support of whose research ,i am able to apply certain facts in my clinic. I have always been saying, as a scientists and scientific minded people, we should not focus on one name ..but the potential of the probable curative techniques.. If we do not discuss, experiment, share,suggest...explore...how can we reach anywhere close to finding better solution for MPB treatments. Am i doing anything wrong, by trying my best to talk ,discuss,share new approaches of treatments with people like Jahoda, gerd,michael,rose,fuchs,christiano, stenn,washniek,rajesh,tyoshima etc.. including some well educated and intelligent forum members/moderators across various forums.... Trying my best ,to get the experts... to work together as a team... Do not worry, you will have independent scientific studies begining shortly and posted on the forums... I hope you are aware, i am starting case studies with few nw7 ...conversion to nw2 with hair doubling. Mr joshi is the first patient whose cells were taken for culture on 5th october 2013.. hopefully as i am back from ishsrs tonight to mumbai..will start his doubling this week. I wanted to share my ishrs visit with you all,but reading your comment , i lost the enthusiasm to post the same... Tom and boldy(my patients from forum) were also there with at the ishrs at san fransisco, ask more info from them... I did discuss few important findings and each others work, with Jahoda at ishrs.

Tom is a forum member in 3 other forums and he is also a ex-freach forum co-ordinator. Dear Friends/critics Kindly find day 100 pics of tom Tom had in vitro donor doubling of 1119 grafts. He also had denovo doubling of approx. 1400 grafts on day 100. These 1400 grafts were taken from the sides and few from the back of the scalp. After three months recipient shows around 350 bisected grafts regrowing after they were mostly shed in first 2 months same like FUE shedding and regrowth. More than 90% bisected at the recipient were upper 3/4th of the follicle without the major hair bulb or hair root. The macro pictures of day 97 are in the camera of Dr. Mwamba, since I was not there in the early morning. Dr. Mwamba technician have stayed back at my clinic. She will provide us with those macro photos, when she will be back at her Belgium clinic. Donor regrowth looks good to me in general. Will do the counting when I have time, better if 1 of u do it. Donor regrowth is good because it is non AGA scalp and predominantly the hair bulbs were implanted there. Recipient regrowth is bonus of in vitro doubling upper part of the hair follicles were implanted. Both donor and recipient received progenitor multiplied stem cells( CD 200+, ALPHA 6 INTEGRIN, CD 34 +CELLS), 2D DP culture and very little 1 ml of 3D spheroid DP AGGREGATES + GROWTH FACTORS + A CELL LIKE ECM. We also did approx. 30 grafts in vitro doubling on Dr. Mwamba staff for patch test who was NW7 slick bold with curly hairs, he will come back after 6 week for multiplied stem cells, 2D CULTURED DP CELLS AND 3D SPHEROID AND DP AGGREGATE + GROWTH FACTORS. I really liked Dr. Mwamba’s work and him as a person, I shared my doubling technique and he shared his micro FUE technique with me. I cant disclose much about our future potential joint venture at EUROPE. Yes he is the most expensive hair transplant surgeon in EUROPE , charges 7 -10 euro /graft. Dr. Mwamba and I will meet again in CHINA at worlds one of the biggest stem cell congress and hopefully at SAN FRANCISCO this October 2013. If u Are not satisfied with the pictures I can take them again, tom will be leaving on 8th September after he receives his TOPICAL NANO solution of growth factor. We are expecting after 1 year regrowth of minimum 90% grafts out of 1119 + 1400 =2519 IN VITRO AND DE NOVO DOUBLING GRAFTS at his hair lines and temples, anything better is bonus. And 2519 grafts regrowth at donor anything better is bonus. Also note the regrowing grafts at donor and recipient are thick like terminal hairs and not lesser diameter or thin hairs even in 3 months. Kindly find the macro pictures below.. Kindly find the Random video microscopic pictures DONOR DAY 0.. Kindly find the Random video microscopic pictures DONOR DAY 100.. Kindly find the Random video microscopic pictures RECEPIENT DAY 0.. Kindly find the Random video microscopic pictures RECEPIENT DAY 100.. Kindly find below DONOR macro pics of DAY 0 AND DAY 100 around toms scar for comparison, yes after the day 0 shaving on day 1 little more shaving was done to extract more grafts. You can compare the donor density at day 0 and day 100 in general. click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/A.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/b.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/c.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/R1.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/R2.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/R3.jpg Donor Day0 click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Large/Bun%201%20L%20to%20R/B1-B.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Large/Bun%201%20L%20to%20R/B1-F.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Large/Bun%201%20L%20to%20R/B1-G.jpg Donor Day 100 click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/1.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/2.jpg click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/3.jpg Recipient day 100 at hairline,hence also little longer hair. click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/4.jpg Recipient day 100,remeber recipient wa slick bald on day zero. click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/5.jpg Recipient day 100,remeber recipient wa slick bald on day zero. click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/6.jpg Recipient day 100,remeber recipient wa slick bald on day zero. click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/7.jpg Recipient day 100,remeber recipient wa slick bald on day zero. click link below to see enlarged close up of the above pic http://www.drnigams.net/images/Tom/Day100/Large/8.jpg

Naresh, You can skype and talk to me skypeid dr.nigams, I hope this will save both your effort and time. The new site is under construction,hence only home page is visible. But as told to gary, i will reload the old site , for you all , so that you can see what you wish. You can mail me and i will mail you the numbers of the ..50%.. you are asking..talk to them , meet them..and only than decide...

Gary, Since the new site is under construction,we have only the home page visible as of know, but specially for you all, i will get the old site uploaded back ,so that you can view the details you wish to see. QUOTE=Gary1911A1;2353872]Dr. Nigam's Site.

Theoretically... yes...but can confirm only as the time pass and we follow ..more and more cases..

Noah, As on today..i have only harvested a particular graft twice..and yes i see approx.90% regen on the second harvest... Kindly review the invivo and invitro doubling pics again.. http://www.drnigams.net/images/RTN/Day53/Large/Donor/Donor-countline.jpg http://www.drnigams.net/images/RTN/Day53/Large/Recipient/Recipient.jpg Pics on the left are of 15graft patch test with invitro hair doubling and pics on the right are of invivo donor doubling around birthmark....

I think you misunderstood,the recipient regen is not 5 to 10%,.... the recipient regeneration is between 90-100% as on today and would vary case to case by approximately 5-10%. As on today, I can speak only about my 8/9 months results which I have seen,... as the time will pass, I can confirm is it possible to repeat donor doubling for how many repeat procedures. theoritically repeat extraction of a regrown follicle of the donor should not have any problem for at least 2-3 times which will solve our problem of NW7. and as months and years pass, we will have documented outcome of the procedures, for us to review and analyze better. as per thickness of the follicle is considered, I don't see any difference from the FUE implanted grafts, the reason being the added stem cells, dp cells, dp culture to repair the bisected follicle quickly and to its original diameter.

Noah, 1)The waiting period ,between the donor doubling surgeries,depends on case to case. For example,if you are a nw6/7,with the number of available grafts at donor scalp(back and sides),arel approx.8000 .... we can remove 4000 grafts in one session of 4 days . in the next session after 6weeks,we will extract the other remaining 4000 grafts( not the regrown grafts of the 1st session). We can repeat this process after 6 months ..to extract 4000 more graft from the regrown grafts of the1st session, at the donor. Regen rate may vary between approx. 5 to 10% at recipient, but will be consistent at the donor since the donor is a non aga scalp. Thus in 6 months a case of nw6/7 can potentially get new 12000 grafts at the recipient and same at the donor. The data ,i am providing you is of my 8/9 months experience with doubling... since we started offering doubling to our patients at our clinic... after small test procedures. We are documenting and following up... three recent cases... of nw7 for doubling of 12000 grafts at the recipient,will update the status of these cases on HRN shortly.. As the doubling has considerably improved in technique, since we started ...i can give you more data on the result with new protocol, in 6months to 1year. NW5/4/3 will need lesser sessions...but i always prefer two sessions for density..as anyway the person has to take multiplied stemcells and dp culture after 6 weeks... 2)I have not yet done a case what you are mentioning.. you have also to take into consideration.... patients overall health status,tolerance to local anesthesia.. .physical/ mental preparedness to do 3/4 sessions by a nw7. I will post what i have posted on other forum...a case of hairline and temples by donor doubling...the patient is an independent member of the forum.. For hairline design and density...we have the new protocol without recipient incisions..but graft implantation with special needle and syringe...thus less trauma,less swelling,hence more density and better survival of the grafts.. We will launch ..world's first image guided hair transplant...with follicle ultrasound..thus all techniques of HT will receive EYES.as you are aware fue is a blind procedure and fut is also partial blind procedure..

Good question,Future-HT-Doc, We started,offering donor doubling to patients 8/9 months ago..after observing the hair growth at donor and recipient..in a miniclinical trials at our clinic. As you must have observed,we are constantly working to improve the same. My observation is.. At the donor scalp which is an non aga scalp,the effect of implanting bisected grafts with progenitor and multiplied stemcells with dp cells and dp culture...is excellent,with additional hair follicles than what we started before the procedure.. in contrast to invivo , which is just bisection and implantation..we have the advantage of injecting activated stemcells.dp cells and dp culture...to repair the bisected follicle.. Yes, the recipient,is comparitively thinner to donor regenerated hair..initially ,specially if the recipient is slick bald(not so in thinning scalp), but by 5/6 months the diametre of recipient hair matches the fue implanted hair, may be because of addition of multiplied stemcells injections after 6 weeks.. With my new technique and the level of bisection ..i am confident..results would improve ..significantly.. I would also introduce shortly..a medical prescription..to be followed for 3 months window period..when a person goes for a doubling ,to reduce androgens and microinflammatory fibrosing irritants at the recipient aga scalp...so that the new grafts get better environment to grow..during early phase of repair.. Newer things to come in coming months is world's first high frequency ultrasound to give eyes to HT/doubling.. Implantation by injections in the recipient, to avoid incisions at the recipient..thus adding lot of benefits like..higher density,less trauma,hardly any microscarring,no swelling,and the implantations will be injections..as the smaller fragmnt of bisected graft, is tiny enough..to be placed in a specially designed needle..with syringe.. But my real focus is pure hm and dp cultured injections...CELLULAR HAIR IMPLANTS...doubling is an extension of ht..but yes it is a significant step forward..to overcome the limited donor problem.. as pure hm gets support from fragments of hair follicle in doubling..rather than forming a denovo hair follicle in pure hm..

Viren, kindly mail me at dr.rahul1970@gmail.com once i know your real name and details..i will get back to you...

Question mark , Yes, i welcome scrutiny to the most detailed possible way....it helps improve and document work. Regards gambhir,yes he is a brand ambassador of a clinic, as an endorsement but gambhir and his brother had come for hair treatment with my director dr anjali,so did bollywood stars.. sonu nigam and sanjay dutt who got their transplant done by her and the staff siraj...PICS are with my director dr anjali with them. So it should be clear now... SEHWAG is a brand ambassador of a clinic,but tmr if he comes to my clinic for some other(stemcell,doubling,dpinjections...as you are aware, he is still bald) procedure..both the clinics can show ..them as their patient.. Scrutiny should be specific...i will provide you specific explanation..and not hearsay.... A true comment..as a grievance is, when a patient puts his real name and gives proof of receipt..that he was treated with the particular clinic. Any innovator, who is a potential threat to anyone's existing business,or is about to change the category itself ..will be envied by them..it is natural . And please read carefully ..and let me know even in their comment...has anyone claimed to be my patient..for hair transplant..there was one and i spoke to him..he will visit me for free touch up for density..

Day 53 Update In-Vitro Doubling of 15 G patch test @Dr. Nigam's Dear friends, Kindly find Day 53 update of donor and recipient of 15 graft patch test of in-vitro hair doubling along with tattoos marks and one shot video. results Donor - On Day 0 total 23 follicles were extracted from 15 grafts (Single - 8, Double - 6 & Triple - 1) On Day 53 by Videoscopic & Macro images of Donor shows - 37 follicles as against 52 follicles on Day 18 may be some must have gone into shedding or some may have not regenerated. Although still better than 23 follicles which were extracted from the donor which mean the gain of 14 follicles at the donor which means as on today (Day 53) regeneration is 160%, may become less or more, actual donor regeneration will come to know in 6 to 9 months. You must be wondering how come more than 100% regeneration that is becuase of stem cells, dp cell, growth factor were injected into the donor and recipient. You can clearly see even after using large punch to extract the graft from the donor like in FUE...... THERE IS NO WHITE DOT OR SCAR MARK. This proves if FUE doctors start using ECM, concentrated PRP and / or growth factor, stem cells, DP cells, visible FUE scar can be avoided. As the above have important role in wound healing. Hence no white dot or scare. Recipient shows 23 follicles as against 38 follicles on Day 18 but still 100% recipient regeneration as 23 bisected follicle were implanted at the recipient, may be the 15 follicles is not seen on Day 53. May be they must have gone into shedding or they must have not regenerated. Actual recipient regeneration will be known after 6 to 8 month. observe the pigmentation and thickness of Donor and Recipient re-growth to analyze the diameter of new hair. WCHR 2013, Edinburgh, UK, had 2 paper presentation on unlimited hair transplant. One Japanese paper were shown which was earlier confirmed by Jahoda that ........ Freshly isolated DP cells which is trichogenic, if implanted at the base of upper half of the amputated / bisected follicles, new dermal papilla or new follicle with similar characteristic of the donor will be created. Another study confirm that upper half of the hair follicle if amputed and extracted with outer root sheath stem cells minimum and great if extracted with matrix stem cells can create it's own dermal papilla. The study also confirmed that the lower dermal papilla when imputed from its upper half of the follicle can create it's own shaft and rest of the follicle. Cotsarelis had published one paper called "De-Differentiation of stem cells" in which bulge stem cells (epidermal) can become matrix germ stem cells and dermal stem cells and similarly dermal stem cells can become epidermal stem cells if required, this is called stem cell De-Differentiation. With my experience of 4 to 5 month of in-vitro and in-vivo technique, as on today I come to conclusion that in-vitro hair doubling or donor doubling will give higher donor & recipient regeneration with better quality of hair than in-vivo donor doubling. since in in-vivo donor doubling the major issues is that its a blind technique and not one can bisect the follicle at the matrix germ cells level or dermal cup sheath level. In-vivo technique has disadvantages.... 1. since it's a blind technique, precise bisection which we want cannot happen 2. Becuase precise bisection cannot happen some extraction cannot suitable hence multiple extraction need to be done 3. Because it is blind technique hence it will be more expensive 4. The hair re-growth at the recipient will be much lesser than the in-vitro technique because only part of upper follicle is being extracted hence hair at the recipient could be thinner. Both In-vitro and in-vivo technique will be scarless but the role of injecting stem cells, dp cells, growth factor, ECM, as per my observation is very important for donor and recipient re-growth and thickness of hair. I remember what Washneik said that in the 1st phase hair multiplication will be used as a adjunct to the hair transplant. This is what I feel too, by the time in coming months we will get consistent result when we will add 3d spheroidal aggregated capsulated, growth factor secretarine trichogenic, good dose of dp cells and / or what Dr. Gerd and Tsuji Lab did that is co-culturing of epithelial stem cells and trichogenic dp cells to create proto hair or micro follicle as named by Gerd for real CELLULAR HAIR IMPLANT or if Histogen succeeds and as believed by Ralf Paus of UK as he told to me in WCHR 2013 that dormant 100000 hair follicles lying also in the slick bald scalp in Telogen phase with partial fibrosis can be activated with stem cells, DP cells, Growth factors, Noggin, WNT7A, etc. in-vivo. I would advice all the patient of AGA doing or not doing hair transplant, doubling or HM should start with mast cells supressor (Oral or topical) OC-03-01, anti-androgen preferably topical will come in the market, antioxydent like melatonin. Oral good dose of antioxidents and smoker should start L-Cystine and biotin in good doses orally and / or topically to counter the follicle damage of nicotine. By next month I will have topical vascular endothelial growth factor FGF and others which can be applied topically for the medical AGA prescription but these topical gels are not available to the patient directly but only to the bio-tech labs. For prevention of AGA I am working on how to reduce on micro inflammation due to multiplication at and around hair follicle and androgenic TGF - Beta-catenine topical or injectible to reduce mast cell released histamines. Reducing DHT concentration on scalp, countering prostaglandin, interlukine 1 & 6 concentration on scalp and products to up regulate PGE2, insulin growth factor, products to prevent apoptosis of stem cells, products to convert dormant stem cells to progenitor stem cells in-vivo through ioantophoresis, electoporation, nano particulate transdermal targetted delivery, chitosan, etc capsulated vehicle. These medical prescription will also helps my HM results if patient starts this medical prescription, same for the regular transplant. If I had movable photon microscope costing US $1 million, my HM result would have increase significantly. Since with movable photon microscope applied on patient scalp. I can see telogen follicles on the human scalp and inject the stem cells directly into it specially when they are fibrosed, partially or totally, normal stem cells injection could be difficult to them. In movable photo microscope, I can see cells of epidermis and dermis and also upward & downward movement of stem cells. As you remember I had mentioned the use of photon micro-scope in cellular regeneration micro imaging study, same was highlighted by 2 presenters with their paper titled "Imaging in hair generative therapies" Elian Fuchs in her paper presentation "Stem cells in silence and in action" showed upward & downward descent video of moving stem cells in a hair follicle in-vivo Hope fully I have to do some research in Gerd lab in Berlin which is having fixed photon microscope and bio-reactors, etc to find the answer that why HM doesn't response on all the scalp? and we know HM respond on some scalp and in these responding scalp the results are really good and acceptable. Donor Count line Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/Donor-countline.jpg Recipient IMG Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/Recipient.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/1.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/2.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/3.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/4.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/5.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/6.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/7.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/8.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/9.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/10.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/11.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/12.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/13.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/14.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Recipient/15.jpg Donor IMG Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/Donor.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/1.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/2.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/3.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/4.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/5.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/6.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/7.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/8.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/9.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/10.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/11.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/12.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/13.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/14.jpg Click the below link for enlarge image http://www.drnigams.net/images/RTN/Day53/Large/Donor/15.jpg Day 53: video link

Sure,hopefully ,i will be injecting the 3D spheroidal culture of trichogenic DP first injection in august..when the culture gets ready..in the mean time...lot of obstacles to overcome...hopefully.... Future doc,will u be there at ISHRS san fransisco october conference...

Torip, . For medical procedures with newer techniques in medical/surgical therapies by a licensed medical physician/surgeon at his facility ..needs 1) clearance from independent institutional committees for research and independent ethical commitee for research.and registration with regulating body on stemcell research, with submission of patients treatment data yearly. 2) Documentation to support the research and or therapy . 3)Patients consent form ,with details of possible beneficial or any potential side effects known or unknown and documentation supporting such claims. 4) This is permissible at a small scale(for furthering research by licensed physician only) and when no drug is being tested which is planned to be marketed through pharmacy in a packaged form. 5)In india..a licensed physician can compound and dispense a medicine at his clinic and give it to his patient for specific medical condition and need to keep records of the same,and be available for followup care and progress... Such compounded and dispensed medicine cannot be sold in pharmacy and the ingredients should have been bought by FDA registered manufacturers or distributors and the physician should supply the bills when asked for.. We have all the above in place.. And we are using autologous ,that is your own stemcells..not allogenic i.e some one else stemcells. There is a difference why histogen has to undergo clinical trials.. 1)Histogen creates growth factors not from ones own ,that is autologous stemcells but by allogenic stemcells of others.. 2)Histogen plans to sell a drug called histogen serum complex,in the market..we do not intend to sell any drug ...we come under classification of newer medical procedures by licensed physicians... 3)Aderans use autologous stemcell solution for injection on scalp...only difference is they intend to supply the same to other doctors in a large scale market across USA..hence they also need clinical trials ....we can only offer the same to our own patient...at our clinic..with above 1to5 conditions in place. What the video you have posted is offering stemcells for intravenous use which is allogenic(not your own stemcells),which are embryonic, in a country where it is not approved and that to through courier...never heard before... We do not offer embryonic but adult stemcells with potential to differentiate into it's restrictive lineage that is skin and hair follicle.. The above conditions are little relax at a small scale by physician monitored therapies to its own patients from its own clinic ...especially... If it is the case of autologous adult stemcells not allogenic or embryonic and with minimal manipulation of stemcells.. i cannot differentiate them or do major manipulation. infact if i do not multiply and only give activated progenitor cells than regulations are minimal.. as, you take out your own blood and prepare platelet rich plasma and inject it back...to your own scalp..even prp has stemcells of your own bone marrow with your own growth facors...prp is been given worldwide for hairloss... I will start my mini clinical trials for patent purpose, after 6 months when we have the perfect cocktail protocol to have a process with highest possible efficacy known to us at that point of time...not to market stemcells as drug but to have our therapy process patented ... As you are also aware that....aderans with autologous stemcells injections have their safety approval with clearance of first phase of clinical trial ..years back.. Remember ACELL is approved by FDA for wound healing and it is can be used off label by licensed physician for any other purpose...and you are aware it is used in USA by many HT surgeons in hair restoration. Similarly ionoprost is approved by FDA for growing eye lashes..but it is off label also used as hair growth potential on scalp.... THER IS A DIFFERENCE IN NEWER MEDICAL PROCEDURE AND USE OF A NEW DRUG...CLASSIFICATION AND REGULATIOIN... I will publish my results, probably this year end... And our team comprises of bio techs,surgeons ,physicians and have our own biotech lab with our own clinic run by licensed physicians....

As i am occupied and focus on my research work...which is at very exciting stage at present..i will find time to atleast document one case a month with proper pics..i have many pics but they are not of great quality hence i dont post them...

krishonkrush, It was nice meeting you at the clinic..especially since you are from the forum...and your result will be interpreted as independent documented result... Regards your question.... I would advise that if you not in a hurry...than you should start with .. 1)Injections of stemcells,inj of dp cells,injection of growth factor on day 0. 2)We will repeat the same with injections of multiplied epithelial and dermal stemcells from your follicle.and injection of 3d spheroidal trichogenic dp/ds culture on day 45 and day 90. 3)If you get great results ..and that can be predicted within 6 months of day 45 injections..you wont have to do any other procedure... If the results are not fully satisfactory or there is no result..we will have to go for donor doubling.... But although your case is of slick bald scalp..but age is on your side...remember as i told you...with combination treatment of HM and doubling i can make any case even with poor donor from nw7 to nw2..and i showed you few pics..especially the malaysian guy who is near nw6/7..and we are working at his scalp..... You have seen my own results on my scalp in person...

krishonkrush, It was nice meeting you at the clinic..especially since you are from the forum...and your result will be interpreted as independent documented result... Regards your question.... I would advise that if you not in a hurry...than you should start with .. 1)Injections of stemcells,inj of dp cells,injection of growth factor on day 0. 2)We will repeat the same with injections of multiplied epithelial and dermal stemcells from your follicle.and injection of 3d spheroidal trichogenic dp/ds culture on day 45 and day 90. 3)If you get great results ..and that can be predicted within 6 months of day 45 injections..you wont have to do any other procedure... If the results are not fully satisfactory or there is no result..we will have to go for donor doubling.... But although your case is of slick bald scalp..but age is on your side...remember as i told you...with combination treatment of HM and doubling i can make any case even with poor donor from nw7 to nw2..and i showed you few pics..especially the malaysian guy who is near nw6/7..and we are working at his scalp..... You have seen my own results on my scalp in person...

Torip, Your comments are compliments.... to the power of regenerative therapies for cellular hair transplant...when you say it is too good to be true... Whenever breakthroughs happen in any field...... it is but natural and understandable to hear such comments.... It would be better if Krishonkush who had been member of HRN forum, and is from mumbai and have visited my clinic..can be given details of this patient who is from mumbai.. and krishonkrush can confirm , is the good to be true results are ... actually TRUE.. Because i am predicting double this density of hair on this patients scalp in next one year ...as we give him our advance new protocol like dp/dscell injectiojs,growth factor injections and dp culture plus further injectiond of stecells... But let me clear..we are not, as on today consistant with such results for every patient,hence we still use doubling for cases who do not respond well with HM injections..but hopefully by next year we may not have to depend on doubling as majority can be treated with STEMCELL INJ,GROWTH FACTORS INJ AND DP CULTURE INJECTIONS ...HM still needs improvement like.. 1)Iinjections of Trichogenic dp cultured cells , with 3d spheroidal cultures,scaffolds use in culture etc. 2)Injections of aggregated trichogenic dp cultured cells. 3)This patient did not recieve growth factor injections and dp cell injections which is now given at our clinic since last 2 months.. 4)Layered co culturing of epithelial stemcells and dp cells.. I have been wondering that same stemcells injected into some scalps can show the above results...and why avg. result in some scalps and no result in certain other scalps..although the basic structure of scalp follilces and process of stemcell culture is same for all...yes there are genetic and other variations in follilces... When one takes fina or minoxidil..even in that case different scalp follices respond differently..but majority respond positively hence fda clearance for it's claim..

Dear Friends, I am happy to share this pure HM result of 4 months with you. I came to know about this patient day before yesterday. He lives 2 and half hour from my Andheri clinic. To share this post I sent a cab to pick up & drop him for our forum members. Thankfully he agreed for the same. We extracted few grafts from his donor scalp on 7th Jan 2013 and took 3 before pictures on 5th Jan 2013. His procedure has not been undertaken at my main clinic but at one of my mini clinic which is located outskirts of Mumbai. On 7th Jay 2013 he received the 1st shot of progenitor activated stem cell injection and 2nd shot of multiplied stem cell was given in the 1st week of April 2013. I took his picture and video today at my clinic and few pictures were also taken at my mini clinic on 13th May 2013. These picture are not for Dr. Nigam's promotion alone but it is also dedicated to all the researcher who have been working on mice. Other companies which are doing their clinical trials....... as these pictures is a great news for both HT doctors and hair loss community that in very near future we will be able to give consistent results to most of our patients........ As on today not all patients will be as lucky as this guy. I am expecting double or triple of this density by next 1 year because I am yet to give him DP CELL INJECTION, TRICHOGENIC 3D SPHEROIDAL DP CULTURE, 2 MORE INJECTIONS OF MULTIPLIED INJECTION, ONCE A MONTH GROWTH FACTOR and this patient is not on any Finasteride or minoxidil. I humbly would like to answer certain critics who used to laugh at me whenever I say 30 K to 40 K hair follicles generation after HM injection on certain patients, now the proof is in front of you. This is interesting case to followup for next 1 year with more HM procedure to be done on him with new protocols. I do not have his before videoscopic picture but I have taken his videoscopic pictures today. Upto now I have not injected on his temples but as in the coming months I will work on his slick bald temples, let us see what will be the result. Similarly I would like to see that am I able to lower his hair line with HM injection alone? In the morning I have seen one more patient with good result, I will post his pictures after 2 months when his scalp becomes more denser. By the way one French forum moderator who is also a forum member before me on HT and BT is coming on 25th May for HM, DP Cells & Doubling. Another forum member is coming tentatively on 15th July. Such case give us hopes that if certain scalp can get such results then in future it will increase. No despair any more, skeptics please be aware, cure is not far away and it will happen on this forum itself. One bad news abstract submission of presentation at international society for hair restoration (ISHRS 2013) is closed on 30th March 2013 and unfortunately I missed the deadline and hence there are very less chance that I will be presenting my case of scarless HT, HM, DP. Although I will be mailing the same to the scientific community, let’s see if I will be able to post the presentation on time. Otherwise maximum what I can do is to be a part of Q&A discussion at San Francisco. Before Click the below link for enlarge image http://www.drnigams.net/images/Savio/Before/Large/1.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/Before/Large/2.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/Before/Large/3.jpg After 4 months Click the below link for enlarge image http://www.drnigams.net/images/Savio/After/Large/5.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/After/Large/6.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/After/Large/7.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/After/Large/8.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/After/Large/9.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/After/Large/10.jpg Before-After Click the below link for enlarge image http://www.drnigams.net/images/Savio/B-A/Large/1.jpg Click the below link for enlarge image http://www.drnigams.net/images/Savio/B-A/Large/2.jpg Video Link http://youtu.be/H19whebfAKY

With our doubling technique we expect more than 100% regeneration at the donor becuase of stem cells & DP cell injection effect on the bisected follicles and 80% to 90% growth at the recipient as on today. Also note, because bisected follicle needs to be repaired at the first stage and then will go in the shedding phase. Please find below the Day 31 picture The below videoscopic images (before-after) are not of the exact location (general before & after images of donor & recipient are provided) as these images has been sent by the patient from the Malaysia. These images of regeneration at Donor show excellent re-growth with hardly any marks (scarless procedure). ou can compare generally the density of donor & recipient. Once we receive the macro pictures within 2 to 3 days from the patient, we will be able to compare the present picture with before picture. We will definitely convert this NW7 to NW2 in maximum next 2 or 3 sittings once this patient comes back for the further procedure. Kindly see the increase in the density at the recipient although before pictures are of random area and not of the entire scalp. Day 31 - Recipient Growth Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/1.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/2.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/3.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/4.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/5.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/6.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Recipient/Large/7.jpg Day 0 - Kindly compare recipient photo with Day 31 growth in the above pics The black marks in the picture is of marker for reference purpose. Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day0/VS/Recipient/Large/1.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day0/VS/Recipient/Large/2.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day0/VS/Recipient/Large/3.jpg Donor regeneration - Day 31 Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Donor/Large/1.jpg Click the below link to enlarge the above pic http://www.drnigams.net/images/MS/Day31/Donor/Large/2.jpg

Kindly find below the abstract which was discussed at WCHR 2013 Edinburgh which I visited recently and had a interaction with Collin Jahoda on the same. Scientific minded members can discuss it further for hair follicle neogenesis P220 Human hair follicle neogenesis using microvironmentally reprogrammed dermal papilla cells click the below link for enlarge image http://www.drnigams.net/images/poster/Large/1.JPG click the below link for enlarge image http://www.drnigams.net/images/poster/Large/2.JPG CA Higgins, CA Jahods2 and AM christiano 1.3 Department of Dermatology, Columbia University, new York, USA , 2 Biological and Biomedical sciences, Durham University, Durham UK and 3 Genetics and Development, Columbia University, New York,USA Hair Follicle (HF) neogensis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using intact Dp or using caltured DP cells. In contrast , HF neogensis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact5 DP , which are capable of neogenesis , but absent in caltured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis.P220 Human hair follicle neogenesis using microvironmentally reprogrammed dermal papilla cells CA Higgins, CA Jahods2 and AM christiano 1.3 Department of Dermatology, Columbia University, new York, USA , 2 Biological and Biomedical sciences, Durham University, Durham UK and 3 Genetics and Development, Columbia University, New York,USA Hair Follicle (HF) neogensis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using intact Dp or using caltured DP cells. In contrast , HF neogensis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact5 DP , which are capable of neogenesis , but absent in caltured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis.

Dear Members, Kindly find below the Histogen presentation at WCHR 2013, Edinburg 2013 sent to me by their corporate communication. I am not aware what results have they published in Oct 2012 and are these results any different from 2012 presentation or no? Hopefully I & Jonathan Mansbridge, (Chief Scientific Officer, Histogen) will be speaking on phone this week and see what best I can come out with for my hair loss community friends for the earliest access, once I am convince with their results in person. click below link for enlarge image http://www.drnigams.net/images/HTG_Embryonic/Large/1.jpg

Made New...... Star Research Friend's Dr. Nigam with Dr. Gerd Lindner & Ken Washniek, CEO, Aderans, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/1.JPG Dr. Nigam with Mike Philpott, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/2.JPG Dr. Nigam with Dr. George Cotsarelis, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/3.JPG Dr. Nigam with Valerie Randall, Chair person of Scientific Committee, & Dr. Costarelis, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/4.JPG Dr. Nigam with Annika Vogte, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/5.JPG She is focusing on nano and virus like particles to deliver specific molecules to targeted areas in hair follicle in vivo and specifically treating hair stem cells by targeted nano molecular therapies. Dr. Nigam with Elaine Fuchs & Valeria Randall, at WCHR 2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/6.JPG Dr. Nigam with Rajesh Thangapazham at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/7.JPG He is a very promising young researcher and he may become consultant to our Lab shortly. He has presented his paper at WCHR20103 tittled "Enhancing hair follicle neogenesis in bioengineered human skin substitute". His company has a collaboration with Dr. Cotsarelis. He is from Pennsylvania, USA Dr. Nigam with Colin Jahoda, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/8.JPG Dr. Nigam with Kurt Stenn, MD Vice President and Chief Scientific Officer, Aderans Research, at WCHR2013, Edinburgh, UK Click here for large images http://www.drnigams.net/images/Edinb...13/Large/9.JPG Click here for large images http://www.drnigams.net/images/Edinb...3/Large/10.JPG Take aways from the visit... 1)Possibilty of topical finasteride with hydroxypropyl chitosan,orwithnano particles as carrier,or with iontophoresis... 2)3D spheroidal dp culture which lowers scalp dht much more(71.2%) than oral FINA(51.11%) in the same time period and possible without/or minimal the side effects of oral Finasteride. 3) Jahoda corrected me to use dermal sheath cells rather than derml papilla cells for my doubling or HM. 4)Stemcells in the hair follicle in silence and in action..various factors effecting it. 5)Hair doubling now officially proven by independent study in vitro in lab and mouse that a bisected hair follicle with outer root sheath can create its own dermal papilla..if kept in right culture medium...thus supporting our claim of hair doubling posted on forums last month. 6)In the near future 1/2years the nano particle based targetted drug delivery to stemcells in the follicle ,dp,fibrosised parts of follicle will be realty as suggested by Annika Voidt from germany... Will post many more take aways from the world congress on hair research soon...