Senior Member Drnigam Posted May 15, 2013 Senior Member Share Posted May 15, 2013 (edited) Kindly find below the abstract which was discussed at WCHR 2013 Edinburgh which I visited recently and had a interaction with Collin Jahoda on the same. Scientific minded members can discuss it further for hair follicle neogenesis P220 Human hair follicle neogenesis using microvironmentally reprogrammed dermal papilla cells click the below link for enlarge image http://www.drnigams.net/images/poster/Large/1.JPG click the below link for enlarge image http://www.drnigams.net/images/poster/Large/2.JPG CA Higgins, CA Jahods2 and AM christiano 1.3 Department of Dermatology, Columbia University, new York, USA , 2 Biological and Biomedical sciences, Durham University, Durham UK and 3 Genetics and Development, Columbia University, New York,USA Hair Follicle (HF) neogensis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using intact Dp or using caltured DP cells. In contrast , HF neogensis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact5 DP , which are capable of neogenesis , but absent in caltured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis.P220 Human hair follicle neogenesis using microvironmentally reprogrammed dermal papilla cells CA Higgins, CA Jahods2 and AM christiano 1.3 Department of Dermatology, Columbia University, new York, USA , 2 Biological and Biomedical sciences, Durham University, Durham UK and 3 Genetics and Development, Columbia University, New York,USA Hair Follicle (HF) neogensis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using intact Dp or using caltured DP cells. In contrast , HF neogensis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact5 DP , which are capable of neogenesis , but absent in caltured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis. Edited May 16, 2013 by drnigam Link to comment Share on other sites More sharing options...
Senior Member Blake Bloxham Posted May 16, 2013 Senior Member Share Posted May 16, 2013 Very interesting! Thank you for sharing. "Doc" Blake Bloxham - formerly "Future_HT_Doc" Forum Co-Moderator and Editorial Assistant for the Hair Transplant Network, the Hair Loss Learning Center, the Hair Loss Q&A Blog, and the Hair Restoration Forum All opinions are my own and my advice does not constitute as medical advice. All medical questions and concerns should be addressed by a personal physician. Link to comment Share on other sites More sharing options...
Senior Member Drnigam Posted June 9, 2013 Author Senior Member Share Posted June 9, 2013 Viren, kindly mail me at dr.rahul1970@gmail.com once i know your real name and details..i will get back to you... Link to comment Share on other sites More sharing options...
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