mariachi

dr mwamba i have a 2 questions

what was the level of bisection in the first protocol?

and will the use of adipose derived stem cells conditioned

medium will make the bisected follicle grow with same diameter?

thanks

Mariachi,

 

He did, but both the upper and lower part of the bisected follicles produced significantly thinner hair shafts. This brings up an important question: what's better, one thick hair shaft or two thin ones?

 

Growth factors could maybe help, but they are risky. Remember that any element that increases the growth of a follicle can also increase and induce growth of unwanted tissue. For example, vegf does induce new vessel growth and could improve flow to doubled follicles. But what is stopping that same vegf form creating new life sustaining vessel growth to something else, like a tumor?

 

In my humble opinion, we must figure out a way to achieve doubling without growth factors. Risks are simply too high otherwise.

growth factors had been used for a long time, prp fpr example contains many growth

factors , histogen is safe and we all know that histogen contains vegf and kgf,

its all about dose, we need just very low dose

blake,

the science behind donor regeneration is already there, all we need is a talented doctor who can

bring this technique to us

dr marco toscani achieved almost 71 % regrowth for both portions( without the use of growth factors),

and we all know that certains growth factors like vegf induces blood supply formation invivo,

that means more nutrition for the follicles that lead to higher growth rate

as for the caliber of hair , wnt10b increase hair shaft diameter

http://www-hsc.usc.edu/~cmchuong/2014Wnt10b-Dkk1.pdf

also vegf modulates the size of the hair follicles .

I'm pretty sure this failed. A year on and seemingly no sign of any result. On the contrary, the people on various forums who have had hair doubling have had no results.

 

I appreciate that Dr Mwamba apparently looked into this but confirmation either way would be nice. His staff have stopped responding to emails about this.

 

He must know by now whether this works or not...

man dr mwamba is still experimenting with this technique, he said that

he would give a confirmation by the summer of 2015

the protocols require a lot of time and we should wait

12 months after that or even longer because it is a diffrent technique

so we shouldn't disturb him, i know it is hard for waiting but dr mwamba is a trustworthy

person and he will tell us the truth

dr mwamba,

there is a study that recommend that wnt 10b expression increase the hair shaft diameter

http://www-hsc.usc.edu/~cmchuong/2014Wnt10b-Dkk1.pdf

hope that helps

dr wesley,

there is very solid science behind this technique, also the experiment or the trial above

done with the tools of regular fue (which achieve 80% survival rate for the grafts)

but with tools of piloscopy we might get 90% survival rate for both upper and lower

parts of the follicles

the disadvantage is the hair regenerated from bisected follicles is thinner than the

original hair (75% of the caliber of the donor hair) , that's why dr mwamba think

that growth factors would solve this issue

blake,

that's why dr mwamba said that applying of growth factors like fgf 9 would increase the

caliber of regenerated hair , also the chinese scientists from shantou university used wnt5a

in thier experiment

i hope you can try longitude bisection because every half contains dsc cells and dp cells

thanks and i hope the best for you

blake,

did you see the study conducted by italian scientists about donor doubling?

they had almost 70% regeneration rate for both lower and upper half

and they did grow terminal hair with a caliber better than the original hair donor

would be nice to hear your opinion

here is the link

Hair Regeneration from Transected Follicles in Duplicative

Surgery: Rate of Success and Cell Populations Involved

BACKGROUND The use of bisected hair follicles in hair transplantation has been previously reported,

but the capacity of each half to regenerate the entire hair has not been clarified.

OBJECTIVE To evaluate duplicative surgery rate of success and to analyze the cell populations involved

in hair regeneration.

METHODS We screened 28 patients undergoing duplicative surgery. Approximately 100 hair follicles

from each patient were horizontally bisected and implanted. Upper and lower portions were stained for

the known epithelial stem cell markers CD200, p63, b1-integrin, CD34, and K19.

RESULTS Similar percentages of hair regrowth after 12 months were observed when implanting the

upper (72.7 7 0.4%) and lower (69.2 7 1.1%) portions. Expression of CD200, p63, and b1-integrin was

detected in both portions, whereas K19 and CD34 stained different cell populations in the upper and

lower fragment, respectively.

CONCLUSION Duplicative surgery might represent a successful alternative for hair transplantation,

because both portions are capable of regenerating a healthy hair. Moreover, our results suggest the

possible presence of stem cells in both halves of the follicle.

dr mwamba, i found a study that made to evaluate donor doubling rate of success

the study showed that the upper half has a 72% regeneration rate and the lower almost 70%

and there is a photo (12 months post op) in the study which shows terminal hair growing

also the caliber of the hair regenerated was finer than the original donor hair

it would be appriciated to hear your opinion dr mwamba

thank you

have any of you guys heard about that doctor?

he claims 50% donor regeneration

Regenerative FUE Hair Transplant by Dr. Amir Yazdan M.D. Orange County & Las Vegas Using ACell & PRP

seems to be like gho's technique

thanks dr mwamba

what about bisection of the lower part of graft as dr gardner said?

i mean , extract a follicle and bisect the lower part only into two parts ,one has dsc cells

and the another has dp cells

by the way dr mwamba , is dr nigam attending the workshop with you next month?

thanks dr

blake,

i have a question,

according to bisection method what are the stem cells in the upper half ?

because dr nigam posted a pic 3 months ago show that the upper half of bisected

follicles growing after shedding on dr nigam first patient(and i think dr mwamba saw that too)

and he (dr nigam) said that the outer root(implanted at donor) must grow new follicle

i think if follicle bisected into two parts: one part has dp cells and the another has dsc cells

it would be great idea

i think dr mwamba that there is a solid science behind this technique but i have a question

is applying growth factors and stem cells necessary to generate hair in the bisection method?

because the hair follicle when bisected every half has the ability to regnerate hair depending on

its own cells

dear dr mwamba

this is dr gardner's(prof jahoda team) opinion regarding (invitro bisection method)

"The best way I could think to do this would be to isolate the DP and also the lower dermal sheath, as both of these contain cells capable of generating a new follicle, although I suppose cutting the lower region of the follicle in two would work as well.

But, this would be very time consuming and require a lot of donor material meaning in turn that it would be extremely expensive which is why I don't think anyone is going really take it further"

dear dr mwamba

this is dr gardner's(prof jahoda team) opinion regarding (invitro bisection method)

"The best way I could think to do this would be to isolate the DP and also the lower dermal sheath, as both of these contain cells capable of generating a new follicle, although I suppose cutting the lower region of the follicle in two would work as well.

But, this would be very time consuming and require a lot of donor material meaning in turn that it would be extremely expensive which is why I don't think anyone is going really take it further"

thanks blake

i agree with you that dr mwamba should do a test without adding any growth factors or any injection

we want this technique (if worked) to be performed in europe or north america

he was supposed to present de novo doubling (apply dp cells , growth factors ,ecm) to reduce white dots

and the hair doubling technique(bisection of the graft)

but the thing that confuses me that dr mwamba said that his test patient didn't get any results after 5 month post op(bisection of graft) while dr mwamba saw the bisected grafts growing at the head of dr nigam patient 3 months post op

is it possible that the telogen phase differ from person to another?

blake

desmons just mentioned that dr nigam will not present his abstracts in the conference

what do you think about that?

appreciated dr mwamba

do you mean dr that the test failed? bad news indeed

dr mwamba , can you update us about your test patient (nw7)?

I think regeneration is appearing less and less likely. Dr Mwamba seems (understandably) reluctant to comment on anything further at this stage. Of course its still early days but judging from the damming comments, failed cases (tom), and disappearance of patients it doesn't look great.

well, tom himself said that dr mwamba made a hair count in brussles after the de novo procedure "second one " and there was 100 % donor regeneration

but the situation regarding in vitro doubling is confusing

thanks dr mwamba , i really appreciate your efforts for the community, could you kindly dr mwamba tell us if there was any donor

regeneration for dr nigam patient (first) and also for your patient (slick nw 7) ?

Sorry to bother you dr mwamba

thanks

dr mwamba, can you give us an update?