mariachi

dr mwamba i have a 2 questions what was the level of bisection in the first protocol? and will the use of adipose derived stem cells conditioned medium will make the bisected follicle grow with same diameter? thanks

growth factors had been used for a long time, prp fpr example contains many growth factors , histogen is safe and we all know that histogen contains vegf and kgf, its all about dose, we need just very low dose

blake, the science behind donor regeneration is already there, all we need is a talented doctor who can bring this technique to us dr marco toscani achieved almost 71 % regrowth for both portions( without the use of growth factors), and we all know that certains growth factors like vegf induces blood supply formation invivo, that means more nutrition for the follicles that lead to higher growth rate as for the caliber of hair , wnt10b increase hair shaft diameter http://www-hsc.usc.edu/~cmchuong/2014Wnt10b-Dkk1.pdf also vegf modulates the size of the hair follicles .

man dr mwamba is still experimenting with this technique, he said that he would give a confirmation by the summer of 2015 the protocols require a lot of time and we should wait 12 months after that or even longer because it is a diffrent technique so we shouldn't disturb him, i know it is hard for waiting but dr mwamba is a trustworthy person and he will tell us the truth

dr mwamba, there is a study that recommend that wnt 10b expression increase the hair shaft diameter http://www-hsc.usc.edu/~cmchuong/2014Wnt10b-Dkk1.pdf hope that helps

dr wesley, there is very solid science behind this technique, also the experiment or the trial above done with the tools of regular fue (which achieve 80% survival rate for the grafts) but with tools of piloscopy we might get 90% survival rate for both upper and lower parts of the follicles the disadvantage is the hair regenerated from bisected follicles is thinner than the original hair (75% of the caliber of the donor hair) , that's why dr mwamba think that growth factors would solve this issue

blake, that's why dr mwamba said that applying of growth factors like fgf 9 would increase the caliber of regenerated hair , also the chinese scientists from shantou university used wnt5a in thier experiment i hope you can try longitude bisection because every half contains dsc cells and dp cells thanks and i hope the best for you

blake, did you see the study conducted by italian scientists about donor doubling? they had almost 70% regeneration rate for both lower and upper half and they did grow terminal hair with a caliber better than the original hair donor would be nice to hear your opinion

here is the link Hair Regeneration from Transected Follicles in Duplicative Surgery: Rate of Success and Cell Populations Involved BACKGROUND The use of bisected hair follicles in hair transplantation has been previously reported, but the capacity of each half to regenerate the entire hair has not been clarified. OBJECTIVE To evaluate duplicative surgery rate of success and to analyze the cell populations involved in hair regeneration. METHODS We screened 28 patients undergoing duplicative surgery. Approximately 100 hair follicles from each patient were horizontally bisected and implanted. Upper and lower portions were stained for the known epithelial stem cell markers CD200, p63, b1-integrin, CD34, and K19. RESULTS Similar percentages of hair regrowth after 12 months were observed when implanting the upper (72.7 7 0.4%) and lower (69.2 7 1.1%) portions. Expression of CD200, p63, and b1-integrin was detected in both portions, whereas K19 and CD34 stained different cell populations in the upper and lower fragment, respectively. CONCLUSION Duplicative surgery might represent a successful alternative for hair transplantation, because both portions are capable of regenerating a healthy hair. Moreover, our results suggest the possible presence of stem cells in both halves of the follicle.

dr mwamba, i found a study that made to evaluate donor doubling rate of success the study showed that the upper half has a 72% regeneration rate and the lower almost 70% and there is a photo (12 months post op) in the study which shows terminal hair growing also the caliber of the hair regenerated was finer than the original donor hair it would be appriciated to hear your opinion dr mwamba thank you

have any of you guys heard about that doctor? he claims 50% donor regeneration Regenerative FUE Hair Transplant by Dr. Amir Yazdan M.D. Orange County & Las Vegas Using ACell & PRP seems to be like gho's technique

thanks dr mwamba what about bisection of the lower part of graft as dr gardner said? i mean , extract a follicle and bisect the lower part only into two parts ,one has dsc cells and the another has dp cells by the way dr mwamba , is dr nigam attending the workshop with you next month? thanks dr

blake, i have a question, according to bisection method what are the stem cells in the upper half ? because dr nigam posted a pic 3 months ago show that the upper half of bisected follicles growing after shedding on dr nigam first patient(and i think dr mwamba saw that too) and he (dr nigam) said that the outer root(implanted at donor) must grow new follicle i think if follicle bisected into two parts: one part has dp cells and the another has dsc cells it would be great idea

i think dr mwamba that there is a solid science behind this technique but i have a question is applying growth factors and stem cells necessary to generate hair in the bisection method? because the hair follicle when bisected every half has the ability to regnerate hair depending on its own cells

dear dr mwamba this is dr gardner's(prof jahoda team) opinion regarding (invitro bisection method) "The best way I could think to do this would be to isolate the DP and also the lower dermal sheath, as both of these contain cells capable of generating a new follicle, although I suppose cutting the lower region of the follicle in two would work as well. But, this would be very time consuming and require a lot of donor material meaning in turn that it would be extremely expensive which is why I don't think anyone is going really take it further"