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Lion

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  1. I don't know if it's the other ingredients in the nizoral shampoo besides ketoconazole that might be to harsh on some people hair to cause it to dry, shed,etc but ketoconzole has been shown to thicken, regrow, and maintain some people hair as well. Look at the following small study I got from another hair loss site. I'm sure it been posted but I'll post just to show what it can do as well. Just to be safe, I'll just use it every 3 or 4 days. Japanese study nizoral CREAM ie Ketoconazole ( with pictures ) Ketoconazole (KCZ), an imidazole anti-fungal agent, is known to be effective for the treatment of seborrheic dermatitis and dandruff. In addition, 2% KCZ shampoo was found to improve hair density and the size and proportion of anagen follicles in androgenetic alopecia (AGA) [1] and, in combination with finasteride, to have an additive effect for AGA. Ketoconazole (KCZ), an imidazole anti-fungal agent, is known to be effective for the treatment of seborrheic dermatitis and dandruff. In addition, 2% KCZ shampoo was found to improve hair density and the size and proportion of anagen follicles in androgenetic alopecia (AGA) [1] and, in combination with finasteride, to have an additive effect for AGA [2]. Recently, it has been reported that topical application of KCZ stimulates hair growth in C3H/HeN mice [3]. However, whether topical KCZ is effective enough to improve the clinical appearance of AGA is not yet clear. We therefore carried out an open trial of topical 2% KCZ lotion (Nizoral?®) in combination with shampoos. Furthermore, to identify the mechanism, which can explain the clinical effect of KCZ on AGA, we performed transient transfection assays using CV-1 cells transfected with androgen receptors (AR). The six Japanese males from 23 to 51 years old were enrolled in this study with their written informed consent. They presented with grade II vertex to IVa AGA according to the Hamilton??“Norwood classification [4]. The subjects applied topical 2% KCZ lotion (Nizoral?®) almost every day during or immediately after hair washing with their own unmedicated shampoos. When they revisited our clinic every several months, clinical pictures were obtained to determine the efficacy of the treatment. Two of the men, one 23 years old with grade II vertex and the other 25 years old with Va AGA, showed remarkable hair regrowth after 6 and 10 months, respectively (Fig. 1). The 23-year-old male stopped using KCZ and 3 months later hair loss recurrence on the vertex was noted (Fig. 1c). When he started using KCZ again during shampooing, hairs on the vertex grew again after 3 months (Fig. 1d). These findings constitute evidence of the clinical efficacy of KCZ for AGA. A 41-year-old male showed a slight increase in vertex hair growth after 1 year. Other three of the men, 31, 38 and 51 years old did not show significant improvement. These findings suggest that topical KCZ with shampoo can be effective for some males with AGA. Fig. 1. A 23-year-old Japanese man who used 2% ketoconazole (KCZ) lotion during shampooing everyday. Six months later, hair regrowth was attained (b) in comparison with the pre-treatment condition (a). Suspension of use for 3 months, however, caused recurrent hair loss ©. Renewed use of KCZ induced renewed growth of vertex hair (d). A 25-year-old Japanese man with AGA (e) applied 2% KCZ lotion immediately after shampooing everyday. Ten months later, hair regrowth was apparent (f). To identify the mechanism, which can explain the effect of KCZ on AGA, we performed transient transfection assays using an androgen-responsive synthetic promoter for CV-1 cells transfected with AR. At 50??“70% confluency in a 24-well plate, the CV-1 cells, maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, were transfected with the transfection reagent Fugene-6 (Roche Diagnostics Corp., Indianapolis, IN, USA) according to the manufacturer's instructions. For luciferase assays, we transfected 0.1??g of the reporter plasmid, pGL2-GRE3-bG-luc [5], 12.5ng of pCI-neo-BamX-AR(Gly23) [5] and 0.2??g of the pRL-CMV vector, the Renilla luciferase control reporter vector driven by the CMV immediate-early enhancer/promoter, as the internal controls. At 24h after transfection, we added fresh DMEM supplemented with 10% charcoal-treated fetal bovine serum with methyltrienolone (R1881, a stable synthetic androgen) or ethanol as a mock vehicle. The cells were also treated with KCZ (Janssen, L.P., Titusville, NJ) or ethanol as a mock vehicle. At 48h after transfection, the cells were harvested for luciferase assays. Luciferase activities were measured with a luminometer using the Dual-Luciferase?„? reporter assay system (Promega, Madison, WI). The results were summarized from three independent sets of transfections and presented as mean?±S.D.; statistical significance was tested with Student's t-test. The results demonstrated that 10 or 20??g/ml KCZ reduced luciferase activity to 67.5% (p<0.01) or 49.9% (p<0.03), respectively, reflecting its suppressive action on AR (Fig. 2). This finding suggests that KCZ improves AGA through the suppression of AR activity. Fig. 2. KCZ effect on R1881-induced AR transcactivity in CV-1 cells transfected with AR. We used the transfection reagent Fugene-6 (Roche Diagnostics Corp., Indianapolis, IN, USA) to transfect 0.1??g of the reporter plasmid, pGL2-GRE3-bG-luc, 12.5ng of pCI-neo-BamX-AR(Gly23) and 0.2??g of pRL-CMV vector (lanes 1??“6) into CV-1 cells cultured at 50??“70% confluency in a 24-well plate. The infected cells were treated with 10??’9M R1881 (lanes 2??“6) or ethanol as a mock vehicle (lane 1). After overnight incubation, KCZ at the indicated concentration was added to the cultures. The activities of the various reporter genes were compared with the luciferase activity in the presence of R1881 and the absence of KCZ (lane 2). Dermal papilla cells are the main targets for androgen in hair follicles, as evidenced by immunohistochemistry [6] and reporter assays [7] for the detection of AR. Deep penetration of KCZ into dermal papilla is therefore necessary to realize the suppressive action on androgen in vivo. The use of KCZ in combination with detergent containing shampoos in this study may enhance KCZ penetration. On the other hand, a recent study demonstrated that KCZ stimulates hair growth in mice [3], suggesting that its effect on hair is androgen independent. To summarize, KCZ may exert its effect on AGA in both an androgen-dependent and -independent manner. References return to Article Outline [1]. [1]Pierard-Franchimont C, De Doncker P, Cauwenbergh G, Pierard GE. Ketoconazole shampoo: effect of long-term use in androgenic alopecia. Dermatology. 1998;196:474??“477. MEDLINE | CrossRef [2]. [2]Khandpur S, Suman M, Reddy BS. Comparative efficacy of various treatment regimens for androgenetic alopecia in men. J Dermatol. 2002;29:489??“498. MEDLINE [3]. [3]Jiang J, Tsuboi R, Kojima Y, Ogawa H. Topical application of ketoconazole stimulates hair growth in C3H/HeN mice. J Dermatol. 2005;32:243??“247. MEDLINE [4]. [4]Norwood OT. Male pattern baldness: classification and incidence. South Med J. 1975;68:1359??“1365. MEDLINE [5]. [5]Inui S, Nakao T, Itami S. Modulation of androgen receptor transcriptional activity by anti-acne reagents. J Dermatol Sci. 2004;36:97??“101. Abstract | Full Text | Full-Text PDF (135 KB) | MEDLINE | CrossRef [6]. [6]Itami S, Kurata S, Sonoda T, Takayasu S. Interaction between dermal papilla cells and follicular epithelial cells in vitro: effect of androgen. Br J Dermatol. 1995;132:527??“532. MEDLINE [7]. [7]Inui S, Itami S, Pan HJ, Chang C. Lack of androgen transcriptional activity in human keratinocytes. J Dermatol Sci. 2000;23:87??“92. Abstract | Full Text | Full-Text PDF (106 KB) | MEDLINE | CrossRef
  2. Hey Bill, Thanks for answering my question. I'm planning to add nizoral to my hair loss regimen(finasteride)since if I'm not mistaken in a study they found that Nizoral increased hair thickness and decreased hair shedding. I'm going to use it every 3 days. I'm not expecting much from it but if it can thicken my hair up even a little bit or help me maintain what I have, I'll be happy with that. I also have oily skin & hair so it will be a bonus if nizoral can help with that as well.
  3. Hi, I remember reading that nizoral post HT wasn't recommmended, so I would like to know when can a person start using Nizoral again post-op and be safe? Thanks
  4. Regarding the FUE cost being lower with neo graft; when it first came out I e-mailed one of the doctors that was using NEO Graft,Dr.Bauman. I asked him how much FUE with neo graft will cost per graft and although I honestly don't remember the exact price he gave me, it was $12 or $15 per graft. Don't know how much he use to charge for FUE pre-neograft but the prize he gave me was a rip off to me.
  5. Just wanted to see if there was any update regarding Dr. feller performing BHT with PRP. But since no one replied; I'm assuming that there's no update
  6. I remember reading that Dr. Feller was thinking of performing Body to scalp HT with PRP soon. Any new news regarding this topic?Thanks!
  7. I believe most of the hair that were going to grow,grew already. What I'm expecting & hoping is for my HT hairs to thicken up enough to make a cosmetic difference. At the moment the thinness and scalp visibility of my HT hair & area looks like if I was balding.Thanks for your reply.
  8. Hi, I'm around 5 month post-op. Although the hairs are growing in, They still not really cosmeticaly making a difference. My scalp is still visible on the area I got my HT, which was on my hairline.I know I'm still early in the process but around what time post-op Will I see thickness, and a cosmetic difference?Thanks
  9. Hi, I'm around 5 month post-op. Although the hairs are growing in, They still not really cosmeticaly making a difference. My scalp is still visible on the area I got my HT, which was on my hairline.I know I'm still early in the process but around what time post-op Will I see thickness, and a cosmetic difference?Thanks
  10. I have experienced something similar as you. I also experienced a uneven growth of HT hair thickness. Some HT hair appeared to grow thicker than others.
  11. Did They actually do a clinical trial on it? Interesting. If they did a clinical trial & it was realiable, & also proven to be effective and you interested, it wont hurt to try it. however the best proven option to halt your hair loss is propecia.
  12. Did They actually do a clinical trial on it? Interesting. If they did a clinical trial & it was realiable, & also proven to be effective and you interested, it wont hurt to try it. however best proven option to halt your hair loss is propecia.
  13. Did They actually do a clinical trial on it? Interesting. If they do a clinical trial & it was realiable, & also proven to be effective and you interested, it wont hurt to try it. however best proven option to halt your hair loss is propecia.
  14. I just wanted to know. I made an appoinment with a dermatologist to get a prescription but thanks anyways.
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